African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2281

Full Length Research Paper

Evaluation of various functional skin parameters using a topical cream of Calendula officinalis extract

Naveed Akhtar1, Shahiq-uz-zaman1, Barkat Ali Khan1*, Haji M1, Shoaib Khan, Mahmood Ahmad1, Fatima Rasool2, Tariq Mahmood1 and Akhtar Rasul1
1Department of Pharmacy, Faculty of Pharmacy and Alternative Medicine, The Islamia University of Bahawalpur, Pakistan. 2College of Pharmacy, University of the Punjab, Lahore, Pakistan.
Email: [email protected]

  •  Accepted: 10 January 2011
  •  Published: 28 February 2011


The aim of this study was to determine the effects of newly formulated W/O  emulsion (cream) of Calendula versus its vehicle (base) as control on skin pH, skin melanin, skin erythema, skin moisture content and transepidermal water loss (TEWL).Hydroalcoholic extract of Calendula plant was entrapped in the inner aqueous phase of the W/O  emulsion. Base without active and formulation having 3% extract of Calendula in the aqueous phase were prepared. Samples were stored at different storage conditions that is, 8, 25, 40, 40°C+75% RH for four weeks to predict their stability. The newly formulated base and formulation were applied to the cheeks of 21 healthy human volunteers for a period of 8 weeks. The base showed insignificant (p>0.05) effects while the formulation decreased TEWL however this decrease was statistically insignificant (p> 0.05). Skin moisture content was significantly (p≤0.05) increased by the formulation.The base showed insignificant (p>0.05) effects while the formulation showed statistically significant (p≤0.05) decrease in skin melanın content. Skın erythema was significantly reduced by the formulation. Skin sebum was significantly (p≤0.05) increased by both creams (base and formulation). Both creams were aesthetic with respect to sensory evaluation. The topical non-invasive application of  of Calendula officinalis cream showed a positive rejuvenating effect on human skin. This study will encourage more attention towards research and more conviction towards utilization of herbal medicines.


Key words: Calendula extract, melanin, erythema, skin sebum, TEWL.