African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2199

Full Length Research Paper

Toxicological potential of ethanol extract of stem bark and Croton heliotropiifolius (Euphorbiaceae)

Willams Alves da Silva
  • Willams Alves da Silva
  • Programa de Pós-Graduação em Morfotecnologia, Centro de Biociências, Laboratório de Histomorfometria.
  • Google Scholar
Kristiana Cerqueira Mousinho
  • Kristiana Cerqueira Mousinho
  • Centro Universitário CESMAC, Brasil.
  • Google Scholar
Mary Anne Medeiros Bandeira
  • Mary Anne Medeiros Bandeira
  • Departamento de Farmácia, Universidade Federal do Ceará, Brasil.
  • Google Scholar
Marcos Aurélio Santos da Costa
  • Marcos Aurélio Santos da Costa
  • Programa de Pós-Graduação em Morfotecnologia, Centro de Biociências, Laboratório de Histomorfometria.
  • Google Scholar
Renatha Claudia Barros Sobreira
  • Renatha Claudia Barros Sobreira
  • Programa de Pós-Graduação em Morfotecnologia, Centro de Biociências, Laboratório de Histomorfometria.
  • Google Scholar
Tainá Maria Santos Silva
  • Tainá Maria Santos Silva
  • Programa de Pós-Graduação em Morfotecnologia, Centro de Biociências, Laboratório de Histomorfometria.
  • Google Scholar
José Gildo da Silva
  • José Gildo da Silva
  • Centro Universitário CESMAC, Brasil.
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Sandrine Maria de Arruda Lima
  • Sandrine Maria de Arruda Lima
  • Programa de Pós-Graduação em Morfotecnologia, Centro de Biociências, Laboratório de Histomorfometria.
  • Google Scholar
Luciano Souza de Lima
  • Luciano Souza de Lima
  • Instituto Federal de Educação e Tecnologia de Pernambuco – IFPE/PE, Brasil.
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Roberta M Leite Lima
  • Roberta M Leite Lima
  • Programa de Pós-Graduação em Morfotecnologia, Centro de Biociências, Laboratório de Histomorfometria.
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Sônia Pereira Leite
  • Sônia Pereira Leite
  • Programa de Pós-Graduação em Morfotecnologia, Centro de Biociências, Laboratório de Histomorfometria.
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  •  Received: 29 May 2020
  •  Accepted: 16 July 2020
  •  Published: 31 August 2020

Abstract

Croton heliotropiifolius is an endemic specie in northeast of Brazil. It is renowned for its medicinal properties, larvicidal, insecticidal and cytotoxic activity. This study evaluated the phytochemical screening of ethanolic extract stem bark of C. heliotropiifolius (EECroton) and the toxicological potential in Swiss albino mice. Phytochemical analysis were performed by thin-layer chromatography. The EECroton at 2000 mg/kg (orally) was used for acute toxicity on Swiss albino mice evaluated during a 14-day period. Histomorphometric analysis of liver and renal tissue along with hematological and serum enzyme levels of serum glutamate oxaloacetate transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT), creatinine and urea were measured. The phytochemical investigation of EECroton revealed the presence of coumarins, flavonoids triterpenes and absence of alkaloid, saponins and condensed tannins. The EECroton (2000 mg/kg: o.v.) showed moderate toxicity and the results of serum hematological and enzymatic levels were similar in the respective groups. Histopathological and histomorphometric analyzes confirmed a low of level of toxicity, showing organization of the structural units of the cells, nuclei and sinusoidal capillaries of the hepatocytes as well as renal corpuscles without presenting morphological damage to their organs. These data suggest that the presence of phenolic compound in EECroton demonstrate low order of toxicity.

Key words: Croton heliotropiifolius, medicinal plants, phytochemical, acute toxicity, histomorphometric.