Abstract

The study aims to evaluate the effect of Tribulus terrestris and Andrographis paniculata on body weight, blood glucose, lipid profile and renal protective capability in alloxan induced type-1 diabetic mice. Six groups of five male Swiss Albino mice each, at four weeks of age were used in this experiment. Group-X were fed saline water and Group-Y with combined herbal formulation at a high dose (1 g/kg bwt) to asses toxicity .The remaining four groups were designated into healthy control, diabetic control , diabetic + Amaryl® 800 µg/kg , Diabetic + Formulation 200 mg/kg bwt .The combined formulation improved body weight loss and lowered blood glucose levels significantly (P<0.001) compared to diabetic control group after 8 weeks of treatment. The total cholesterol and plasma triglyceride were decreased at a significant level (P<0.01) compared to diabetic control. However, High density lipoproteins (HDL) and Low density lipoproteins (LDL) levels remained unchanged by combined formulation compared to diabetic control. The combined formulation also lowered the plasma creatinine levels in the formulation treated group compared to diabetic control. Histo-pathological evaluation revealed that combined formulation partially improved renal glomerular sclerosis and hypertrophy, tubular damage and pancreatic β-cells damage. The combined herbal formulation may have antidiabetic and renal protective capability in alloxan induced type-1 diabetic mice.

Key words: Tribulus terrestris, Andrographis paniculata, blood glucose, lipid profile, renal injury, anitdiabetic effects.