African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2281

Full Length Research Paper

Caffeic acid reduces lipid accumulation and reactive oxygen species production in adipocytes

Mariana Brito Dantas
  • Mariana Brito Dantas
  • Clinical and Toxicological Analysys Department, Faculty of Pharmacy, Odontology and Nursing, University Federal of Ceara, Brasil.
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Tiago Lima Sampaio
  • Tiago Lima Sampaio
  • Physiology and Pharmacology Department, Faculty of Medicine, University Federal of Ceara, Brasil.
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Ramon Róseo Paula Pessoa Bezerra de Menezes
  • Ramon Róseo Paula Pessoa Bezerra de Menezes
  • Clinical and Toxicological Analysys Department, Faculty of Pharmacy, Odontology and Nursing, University Federal of Ceara, Brasil.
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Jamile Magalhães Ferreira
  • Jamile Magalhães Ferreira
  • University of International Integration of Afro-Brazilian Lusophony, Brazil.
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Tiago Sousa Melo
  • Tiago Sousa Melo
  • Clinical and Toxicological Analysys Department, Faculty of Pharmacy, Odontology and Nursing, University Federal of Ceara, Brasil.
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Richard Rarison Cavalcante Meneses
  • Richard Rarison Cavalcante Meneses
  • Clinical and Toxicological Analysys Department, Faculty of Pharmacy, Odontology and Nursing, University Federal of Ceara, Brasil.
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Hector Galdino Rodrigues
  • Hector Galdino Rodrigues
  • Clinical and Toxicological Analysys Department, Faculty of Pharmacy, Odontology and Nursing, University Federal of Ceara, Brasil.
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Dânya Bandeira Lima
  • Dânya Bandeira Lima
  • Clinical and Toxicological Analysys Department, Faculty of Pharmacy, Odontology and Nursing, University Federal of Ceara, Brasil.
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Alice Maria Costa Martins
  • Alice Maria Costa Martins
  • Clinical and Toxicological Analysys Department, Faculty of Pharmacy, Odontology and Nursing, University Federal of Ceara, Brasil.
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Maria Goretti Rodrigues de Queiroz
  • Maria Goretti Rodrigues de Queiroz
  • Clinical and Toxicological Analysys Department, Faculty of Pharmacy, Odontology and Nursing, University Federal of Ceara, Brasil.
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  •  Received: 06 June 2018
  •  Published: 22 June 2018

Abstract

Caffeic acid (CA) is a hydroxycinnamic acid, a group of natural organic phenolic compounds used in folk medicine and in foods for the prevention and treatment of obesity. CA is present in a variety of plants, as it is an intermediate in secondary metabolism. It is found in coffee, wine and olive oil. The aim of this work was to study the influence of caffeic acid (CA) on the reduction of intracellular lipid accumulation, intracellular reactive oxygen species (ROS) formation and in mitochondrial transmembrane potential alterations in differentiated 3T3-L1 cells. The pre-adipocyte cell line 3T3-L1 was differentiated using a differentiation cocktail. Cells were treated with CA, and the MTT assay was performed to assess the effect of CA on pre-adipocytes.  The quantification of lipids accumulated within the mature cells was performed using Oil Red O dye. Flow cytometry was used to evaluate the production of ROS through 2',7'-dichlorofluorescein diacetate (DCFH-DA) oxidation and changes in mitochondrial transmembrane potential by Rhodamine 123 dye (Rho123). CA did not exhibit significant toxicity on the 3T3-L1 cell line at the studied concentrations. Caffeic acid causes significant reduction of lipid content in the cells submitted to the post- and co-treatment, being more effective in co-treatment. Caffeic acid results in an absolute decrease in the formation of intracytoplasmic reactive oxygen species. The treatment with caffeic acid protects against oxidative stress caused in the mitochondria by the adipocyte differentiation process. Thus, CA acts on adipogenesis, reducing intracellular lipid accumulation in the 3T3-L1 cells. It reduces intracellular ROS formation and mitochondrial transmembrane potential alterations in differentiated cells.

Key words: Caffeic acid, adipocytes, reactive oxygen species, oxidative stress.