African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2195

Short Communication

Potentialization of antioxidant activity of Piper umbellata (Piperaceae) leaves after their metabolism in Heraclides brasiliensis larvae (Lepidoptera: Papilionidae)

Clécio S. Ramos*, Andra J. L. N. Paz, Telma M. G. da Silva and Tânia M. S. da Silva
Department of Molecular Sciences, Rural Federal University of Pernambuco, Recife - PE, Brazil.
Email: [email protected]

  •  Accepted: 19 June 2012
  •  Published: 29 December 2012


Piper umbellata leaves accumulate the compound, 4-nerolidylcatechol, which is responsible for the antioxidant power of the plant. Surprisingly, in this study we observed a significant increase in the antioxidant activity of P. umbellata leaves after their metabolism on Heraclides brasiliensis larvae. The total phenolic concentrations of the extracts from the leaves and fecal matter (metabolized leaves) measured using the Folin-Ciocalteu reagent, were 14.78 and 27.13 mg GAE/g, respectively. The antioxidant activity, based on 2,2-azinobis 3-ethylbenzothiozoline-6-sulfonic acid (ABTS), was EC50 of 120.10 and 62.50 ug/ml for the leaves and fecal material, respectively. These values were correlated with the total phenolic content.


Key words: Piper umbellataHeraclides brasiliensis, antioxidant, metabolism.