African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2118

Full Length Research Paper

Evaluation of activity exerted by a steroid derivative on injury by ischaemia/reperfusion

Lauro Figueroa-Valverde
  • Lauro Figueroa-Valverde
  • Laboratorio de Farmacoquímica de la Facultad de Ciencias Químico-Biológicas de la Universidad Autónoma de Campeche. Av. Agustín Melgar, Col Buenavista C.P. 24039, Campeche Cam., México.
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Francisco Díaz-Cedillo
  • Francisco Díaz-Cedillo
  • Escuela Nacional de Ciencias Biológicas del Instituto Politécnico Nacional. Prol. Carpio y Plan de Ayala s/n Col. Santo Tomas, México, D.F. C.P. 11340, México.
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Marcela Rosas-Nexticapa
  • Marcela Rosas-Nexticapa
  • Facultad de Nutrición, Universidad Veracruzana. Médicos y Odontólogos s/n, 91010, Xalapa, Veracruz, México.
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ElodiaGarcía-Cervera
  • ElodiaGarcía-Cervera
  • Laboratorio de Farmacoquímica de la Facultad de Ciencias Químico-Biológicas de la Universidad Autónoma de Campeche. Av. Agustín Melgar, Col Buenavista C.P. 24039, Campeche Cam., México.
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Eduardo Pool-Gómez
  • Eduardo Pool-Gómez
  • Laboratorio de Farmacoquímica de la Facultad de Ciencias Químico-Biológicas de la Universidad Autónoma de Campeche. Av. Agustín Melgar, Col Buenavista C.P. 24039, Campeche Cam., México.
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Maria López-Ramos
  • Maria López-Ramos
  • Laboratorio de Farmacoquímica de la Facultad de Ciencias Químico-Biológicas de la Universidad Autónoma de Campeche. Av. Agustín Melgar, Col Buenavista C.P. 24039, Campeche Cam., México.
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Betty Sarabia-Alcocer
  • Betty Sarabia-Alcocer
  • Faculty of Medicine from University Autonomous of Campeche, Av. Patricio Trueba de Regil s/n, Col Lindavista C.P.24090 Campeche Cam., México.
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Isamar Damian-Hernandez
  • Isamar Damian-Hernandez
  • Laboratorio de Farmacoquímica de la Facultad de Ciencias Químico-Biológicas de la Universidad Autónoma de Campeche. Av. Agustín Melgar, Col Buenavista C.P. 24039, Campeche Cam., México.
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  •  Accepted: 03 February 2014
  •  Published: 08 February 2014

Abstract

There are reports which indicate that some steroid derivatives have activity atcardiovascular level; nevertheless, there is scarce information about the effects exerted by the progesterone derivatives on cardiac injury caused by ischemia/reperfusion. In this study, a new steroid (progesterone derivative) was synthetized with the objective of evaluating its activity on ischemia/reperfusion injury. The Langendorff technique was used to evaluate the effect of progesterone derivative on ischemia/reperfusion injury. Additionally, molecular mechanism involved in the activity exerted by the progesterone derivative on perfusion pressure and coronary resistance was evaluated by measuring left ventricular pressure in absence or presence of following compounds; mifepristone, prazosin, metoprolol, indomethacin and nifedipine. The results showed that the progesterone derivative reduces infarct size compared with control. Other results showed that the progesterone derivative significantly increase (p = 0.05) the perfusion pressure and coronary resistance in isolated heart. Other data indicate that the progesterone derivative increase left ventricular pressure in a dose-dependent manner (0.001 to 100 nM); however, this phenomenon was significantly inhibited by nifedipine at a dose of 1 nM (p = 0.05). In conclusion, these data suggest that progesterone derivative exert acardioprotective effect via the calcium channels activation and consequently induces changes in the left ventricular pressure levels. This phenomenon results in decrease of myocardial necrosis after ischemia and reperfusion.

Key words: Steroid, progesterone, left ventricular pressure, indomethacin.