African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2225

Full Length Research Paper

Synergic effect of associated green, red and brown Brazilian propolis extract onto Streptococcus mutans and Streptococcus sanguinis

João Luís Duval Cândido da Silva1, Fernando Freitas Silva1, Thiago Fernandes Machado de Souza1, Wellington Gomes Generoso1, Vladimir Reimar Souza Noronha2, Elizete Maria Rita Pereira2 , Mariana Passos De Luca2,  Sheila Lemos Rago Abreu3 and Vagner Rodrigues Santos4*    
1Graduation Student – Laboratory of  Microbiology, Faculty of Dentistry Universidade Federal de Minas Gerais, Belo Horizonte, Brasil. 2Dentistry Post-Graduation student - Universidade Federal de Minas Gerais- Belo Horizonte, Minas Gerais, Brasil. 3Pharmaceutical Biochemistry -PharmaNéctar® industry – Belo Horizonte- Minas Gerais – Brazil. 4Professor, DDS, PhD, Faculty of Dentistry – Universidade Federal de Minas Gerais.    
Email: [email protected]

  •  Accepted: 24 June 2013
  •  Published: 08 August 2013

Abstract

Propolis is an organotherapeutic product collected by honeybees and has relevant pharmacological properties, highlighting the high antimicrobial activity. This study aimed to evaluate in vitro the synergistic effect between three ethanol extract of different Brazilian propolis samples: green (Baccharis dracunculifolia) (A), red (Dalbergia ecastophyllum) (B) and brown (Copaifera sp) (C) propolis by antimicrobial sensitivity of Streptococcus mutans(ATCC 25175) and Streptococcus sanguinis (ATCC 10557), through the agar diffusion method. Aliquots of each microorganism containing 1.0x106 CFU / mL were inoculated on Mueller- Hinton agar supplemented with 5% dextrose and sterile blank discs containing 20 μL of each propolis sample (A, B, C) and combined (A + B, A + C, B + C, and  A + B + C) were planted on the agar. Tetracycline 30 mg discs and blank discs containing 70% alcohol served as controls. After incubation at 37°C in bacteriological incubator in a 5% COatmosphere for 24 and 48 h the inhibition zones were measured. The results showed that all extracts inhibited the growth of both microorganisms, while the samples (B) and (A + B), were significantly more effective than the others. For samples B and C similar results were observed.

 

Key words: Streptococcus mutansStreptococcus sanguinis, Brazilian propolis, antimicrobial activity, synergism.