African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2184

Full Length Research Paper

Phenolic content and antioxidant activity of crude and fractionated extracts of Pereskia bleo (Kunth) DC. (Cactaceae)

K. S. Sim1, A. M. Sri Nurestri 1* and A. W. Norhanom2
1Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia. 2Centre for Foundation Studies in Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Email: [email protected]

  • Article Number - 880740225465
  • Vol.4(5), pp. 193-201 , May 2010
  •  Accepted: 26 March 2010
  •  Published: 31 May 2010


The total phenolic content of the extracts of Pereskia bleo were assessed by the Folin-Ciocalteau’s method while the antioxidant activities were determined by three different assays, namely scavenging activity on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, reducing power assay and ß-carotene method. In the present study, the ethyl acetate extract exhibited the highest total phenolic content (40.12 mg of GAEs/g of extract) and showed the strongest antioxidant activity in the ß-carotene bleaching assay significantly. Whilst, the hexane extract showed significantly the highest antioxidant activity when determined by scavenging effect on DPPH radicals (EC50 210 µg/ml) and the reducing power assay.


Key words: Pereskia bleo, Cactaceae, antioxidant activity, phenolic content.