African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2285

Full Length Research Paper

In vitro elution and dissolution of tobramycin and gentamicin from calcium phosphate

Che Nor Zarida C. S.1, Fauziah O.1*, Arifah A. K.2, Azfar Rizal A.3, Nazri M. Y.4, Ahmad Hafiz Z.4, Rusnah M.5, Mohd Azam Khan G. K.6 and Hasni Idayu S.7
1Department of Human Anatomy, Faculty of Medicine and Health Sciences, University Putra Malaysia, 43400 Serdang, Malaysia. 2Department of Veterinary Preclinical Science, Faculty of Veterinary Medicine, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia. 3Department of Orthopaedics, Faculty of Medicine and Health Science, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia. 4Department of Orthopaedics, Traumatology and Rehabilitation, Kulliyyah of Medicine, International Islamic University Malaysia, Jalan Hospital, 25150 Kuantan, Malaysia. 5Malaysian Nuclear Agency (Nuclear Malaysia), Bangi, 43000 Kajang, Selangor, Malaysia. 6Faculty of Veterinary Medicine, University Malaysia Kelantan, Karung Berkunci 36, Pengkalan Chepa, 16100 Kota Bharu, Kelantan, Malaysia. 7Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, University Putra Malaysia, 43400 Serdang, Malaysia.
Email: [email protected]

  •  Accepted: 21 October 2011
  •  Published: 29 November 2011

Abstract

This study was conducted to investigate the in vitro drug release characteristics of tobramycin and gentamicin from calcium phosphate. Calcium phosphate beads were loaded with tobramycin and gentamicin separately to form 2 types of antibiotic beads to generate antibiotic drug delivery system for the treatment of osteomyelitis. Tobramycin and gentamicin concentrations were determined spectrophotometrically by measuring the absorbance at 400 nm. The standard graphs for tobramycin and gentamicin concentration versus absorbance reading were prepared as references to identify the concentration of the drugs release after incorporating calcium phosphate over 8 weeks. This study showed that incorporating tobramycin and gentamicin with calcium phosphate provided slow residual release of antibiotic from 30 min to 1344 h (8 weeks) and dissolution of calcium phosphate. In this respect, the drug delivery systems of tobramycin and gentamicin-incorporated calcium phosphate have potential of controlling drug release.

 

Key words: Tobramycin, gentamicin, calcium phosphate, drug delivery system, osteomyelitis.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

Back to Vol. 5 No. 20
Back to articles
  • Articles on Google by:
SUBSCRIBE TO RSS
SUBMIT MANUSCRIPT
CONFERENCES