African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2148

Full Length Research Paper

Tripterine induces apoptosis of human acute myelocytic leukemic cells via up-regulating Fas/FasL and down-regulating NF-κB

Sheng Quan1, Yin-Hai Xu2, Xiao-Ping Xia3*, Jin-Fang Zhao4 and Jie Yan4
1Department of Clinical Medicine, Zhejiang University City College, Hangzhou 310015, P. R. China. 2Department of Clinical Laboratory, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, P. R. China. 3Clinical Laboratory of Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou 310016, P. R. China. 4Faculty of Basic Medicine, College of Medicine, Zhejiang University, Hangzhou 310058, P. R. China.
Email: [email protected]

  • Article Number - 9EA6CD829399
  • Vol.4(7), pp. 431-435, July 2010
  •  Accepted: 16 June 2010
  •  Published: 31 July 2010

Abstract

This study investigated the mechanism underlying the effect of tripterine on apoptosis of human acute myelocytic leukemic cells (HL-60). The apoptosis of HL-60 cells after treatment with triperine of different dosages and durations were determined using flow cytometry and transmission electron microscopy. The expressions of Fas, FasL and NF-κB in the HL-60 cells treated with 1.5 μmol/L tripterine for different durations were measured by flow cytometry. Our results showed treatment with 0.5 ~ 2.5 μmol/L tripterine for 24 h could induce apoptosis of HL-60 cells to different extents (apoptotic rate: 6.13 ~ 36.71%) in which the maximal apoptotic rate was observed after 1.5 μmol/L tripterine treatment for 24 h. In addition, 1.5 μmol/L tripterine could cause gradually increased apoptotic rates in a time-dependent manner presenting typical apoptotic morphology in the HL-60 cells. In the 1.5 μmol/L tripterine-treated HL-60 cells, the number of Fas and FasL positive cells was significantly increased (P < 0.05) accompanied by a markedly decreased number of NF-κB positive cells (P < 0.05), which was in a time dependent manner. Tripterine could effectively induce apoptosis of HL-60 cells and its antitumor mechanism may be related to both the up-regulation of Fas and FasL and down-regulation of NF-κB.

 

Key words: Tripterine, HL-60 cells, apoptosis, Fas/FasL, NF-κB, regulation.

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