Abstract

A selective, rapid and accurate liquid chromatographic method is described for the simultaneous determination of valsartan (VAT) and hydrochlorothiazide (HTZ) in a solid dosage form. The chromatographic separation of two drugs were achieved on Lichrosphere CN column 250 mm × 4.0 mm,5 µm, Merck-India. The mobile phase consisting mixture of methanol, isopropyl alcohol and n-hexane in the ratio of 50:25:25 v/v was delivered at a flow rate of 1.0 ml/min. Detection was performed at 265 nm. Linearity, accuracy and precision were found to be acceptable over the concentration range 40 to120 µg/ml for VAT and 6 to 18 µg/ml for HTZ, respectively. Seperation of VAT and HTZ were achieved in 5 min along with possible degradants. The proposed method can be used for the quantitative analysis of VAT and HTZ in formulation product.

Key words: Valsartan, hydrochlorothiazide, liquid chromatography.