African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2201

Full Length Research Paper

Inhibitory effect of betulinic acid and 3β-acetoxybetulinic acid on rat platelet aggregation

Habila A. J1*, Habila J. D4, Shode F. O2, Opoku A. R3, Atawodi S. E1 and Umar I. A1
1Department of Biochemistry, Ahmadu Bello University Zaria-Nigeria. 2School of Chemistry, University of Zululand, South Africa. 3Department of Biochemistry and Microbiology, University of Zululand, South Africa. 4Department of Chemistry, Ahmadu Bello University Zaria-Nigeria.
Email: [email protected]

  •  Accepted: 21 November 2013
  •  Published: 29 November 2013


Platelet aggregation is one of the major causes of cardiovascular diseases. Our search for bioactive molecules from nature, led to the isolation of betulinic acid (BA) and structural modification of BA to 3β-acetoxybetulinic acid (BAA). Both inhibited blood platelet aggregation induced by thrombin, adenosine diphosphate (ADP) and epinephrine. BAA showed an enhance inhibition of platelet aggregation, in the thrombin-induced platelet aggregation (54.5±0.01 at 1 mg/ml, 63.5±0.17 at 3 mg/ml and 73.5±0.15 at 10 mg/ml; IC50 0.81 mg/ml) which was observed to be significantly (p<0.05) similar to that of the standard aspirin (65.4±0.07 at 1 mg/ml, 72.1±0.03 at 3 mg/ml and 76.5±1.22 at 10 mg/ml; IC50 0.33 mg/ml). The results clearly shows that functional group modification of BA to give BAA led to enhanced activity, hence BAA provides a better option as lead in the search for anti-platelet aggregation agents from nature.

Key words: Anti-platelet aggregation, aggregation inducer, Betulinic acid, acetoxybetulinic acid, Melalueca bracteata.