African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2241

Full Length Research Paper

Antinociceptive and anti-inflammatory effects of the methanol seed extract of Carica papaya in mice and rats

A. O. Anaga* and E. V. Onehi
Department of Veterinary Physiology and Pharmacology, University of Nigeria, Nsukka, Enugu State, Nigeria
Email: [email protected]

  •  Accepted: 11 February 2010
  •  Published: 30 April 2010


The methanol seed extract of Carica papaya (CPE) was investigated for antinociceptive and anti-inflammatory activities in mice and rats. The brine shrimp lethality test of the extract showed LC50 of 106.94 ppm at 95% confidence interval. The extract (5, 10 and 20 mg/kg, p.o.) significantly (p < 0.05) increased pentobarbitone-induced sleeping time by more than 280% compared with the control. Like pethidine (9.1 mg/kg, i.p.), CPE (10 and 20 mg/kg, p.o.) significantly (p < 0.05) decreased the paw licking time in early (0 - 5 min) and late (25 - 30 min) phases of formalin-induced nociception. This antinociceptive effect was more in the late phase than early phase. Also, CP (5, 10 and 20 mg/kg, p.o.) significantly (p < 0.05) decreased the number of acetic acid-induced abdominal contortions by 25, 60 and 64%, respectively. Indomethacin (10 mg/kg, p.o.), CPE (5, 10 and 20 mg/kg, p.o.) showed a typical biphasic anti-inflammatory effect in carrageenin-induced paw oedema in rats. The anti-inflammatory effect though moderate, was dose-dependent and higher in 2 h than 4 h after administration of the phlogistic agent. In conclusion, CPE contains potent bioactive compounds (alkaloids, flavonoids and polyphenols) which showed antinociceptive effect probably mediated centrally and peripherally; and also involving mild anti-inflammatory mechanisms.


Key words: Carica papaya, brine shrimps, antinociception, anti-inflammatory.