The present study aims to evaluate the antitumor, antioxidant and anti-inflammatory activities of two molluscan extracts, Sepia officinalis, ink extract (IE) and Coelatura aegyptiaca extract (CE). The antioxidant activities of both extracts were evaluated using 1,1- diphenyl-2-picrylhydrazyl (DPPH) scavenging and lipid peroxidation assays. The analgesic effects were evaluated using the writhing, hot plate and formalin tests.  Cytotoxicity assay was performed using sulphorhodamine B (SRB) method on hepatocellular carcinoma (HepG2) cell lines. The IE extract exhibited dose dependent radical scavenging activity. The two tested extracts showed inhibition of thiobarbituric acid–reactive substances (TBARAS) at all concentrations, with an IC50 value of 176.77 and 177.23 (µg/ml), respectively. IE and CE extracts showed analgesic action by inhibiting the acetic acid-induced writhing. IE exhibited significant anti-nociceptive actions in mice by increasing the latency period in the hot-plate test. Both extracts significantly decreased the time of paw lickings in both early and late phases.  Furthermore, IE and CE showed cytotoxic activities HepG2 cell lines with IC50 value of 67 and 49.24 µg/ml, respectively. In conclusion, IE and CE extracts have antioxidant, anti-inflammatory and cytotoxic properties and can be considered as promising anticancer drugs.

Key words: Sepia ink, Coelatura aegyptiaca, antioxidant, anti-inflammatory, cytotoxicity.