African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2162

Full Length Research Paper

Inhibitory effect of oxymatrine on the proliferation and vascular endothelial growth factor A (VEGF-A) expression in human lung adenocarcinoma (SPC-A-1) cell

Rui Liang1, Wei Zhang2 and Shoude Jin1*
1Department of Respiratory, the Fourth Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, PR China. 2Department of Respiratory, the First Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, PR China.
Email: [email protected]

  •  Accepted: 25 October 2012
  •  Published: 15 December 2012

Abstract

The aims of this study were to investigate the effect of oxymatrine (OMA) on human lung adenocarcinoma (SPC-A-1) as well as to explore the underlying mechanism. The inhibitory effects of OMA on the growth of SPC-A-1 cells were tested by using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method, apoptosis assay and the expressions of vascular endothelial growth factor A (VEGF-A). OMA with different concentrations had significant inhibitory effects on the growth of SPC-A-1 and could also induce apoptosis. In addition, real time-polymerase chain reaction (RT-PCR) analysis indicated that OMA dramatically reduced the secretion of VEGF dose-dependently. The results suggested that OMA might have the therapeutic application in the treatment of human lung adenocarcinoma cell lung cancer.

 

Key words: Oxymatrine, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), real time-polymerase chain reaction (RT-PCR), vascular endothelial growth factor A (VEGF-A), human lung adenocarcinoma (SPC-A-1).

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