Abstract

The aims of this study were to investigate the effect of oxymatrine (OMA) on human lung adenocarcinoma (SPC-A-1) as well as to explore the underlying mechanism. The inhibitory effects of OMA on the growth of SPC-A-1 cells were tested by using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method, apoptosis assay and the expressions of vascular endothelial growth factor A (VEGF-A). OMA with different concentrations had significant inhibitory effects on the growth of SPC-A-1 and could also induce apoptosis. In addition, real time-polymerase chain reaction (RT-PCR) analysis indicated that OMA dramatically reduced the secretion of VEGF dose-dependently. The results suggested that OMA might have the therapeutic application in the treatment of human lung adenocarcinoma cell lung cancer.

Key words: Oxymatrine, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), real time-polymerase chain reaction (RT-PCR), vascular endothelial growth factor A (VEGF-A), human lung adenocarcinoma (SPC-A-1).