African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2285

Full Length Research Paper

Apoptotic effects of dehydroepiandrosterone sulphate (DHEAS) of the human promyelocytic leukemia cell (HL-60)

Sema Uslu1*, Mehtap Kutlu2, Eda Ozcelik1 and Arzu Iscan2
1Department of Medical Biochemistry, Faculty of Medicine, Eskisehir Osmangazi University, Eskisehir, Turkey. 2Department of Biology, Institution of Science, Anadolu University, Eskisehir, Turkey.
Email: [email protected].

  •  Accepted: 26 March 2012
  •  Published: 04 April 2012

Abstract

Dehydroepiandrosterone sulphate (DHEAS) has a protective role against cancer; however, the mechanism by which DHEAS has this effect remains poorly understood. The purpose of this study was to investigate the potential effects of DHEAS on HL-60 (human promyelocytic leukemia cell). The percentage of cells undergoing apoptosis was analyzed by flow cytometry using Annexin V-fluorescein isothiocyanate staining. DHEAS internalization into HL-60 cells was visualized by transmission electron microscopy. DHEAS could be used to facilitate uptake to specific cancer cells for cancer therapy and diagnosis. Our results showed that the uptake of DHEAS by HL-60 cells were also much higher than that of the control cells. In this study, the mechanism of DHEAS actions against leukemia involves the inhibition of cell proliferation indicating that DHEAS can be useful in the treatment of leukemia.

 

Key words: Apoptosis, dehydoepiandrosterone sulphate, human promyelocytic leukemia cell.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

Back to Vol. 6 No. 13
Back to articles
  • Articles on Google by:
SUBSCRIBE TO RSS
SUBMIT MANUSCRIPT
CONFERENCES