African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2191

Full Length Research Paper

Evaluation of in vitro antioxidant, reducing, lipoxygenase and ACE inhibition activity of polyherbal drug linkus

Hina Rehman
  • Hina Rehman
  • Faculty of Pharmacy, Jinnah Women University, Karachi -74600-Pakistan.
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Zeeshan Ahmed Shaikh
  • Zeeshan Ahmed Shaikh
  • Herbion Pakistan (Pvt.) Ltd., Korangi Industrial Area, Karachi-74900, Pakistan.
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Safila Naveed
  • Safila Naveed
  • Faculty of Pharmacy, Jinnah Women University, Karachi -74600-Pakistan.
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Mahreen Latif
  • Mahreen Latif
  • Multidisciplinary Research Laboratory (MDRL), Bahria University Medical and Dental College, Karachi-75500, Pakistan.
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Khan Usmanghani
  • Khan Usmanghani
  • Herbion Pakistan (Pvt.) Ltd., Korangi Industrial Area, Karachi-74900, Pakistan.
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  •  Received: 29 December 2015
  •  Accepted: 18 February 2016
  •  Published: 29 November 2016

Abstract

Oxidative, reductive, lipoxygenase and angiotensin converting enzyme (ACE) activities are the condition where there is an inequity among concentrations can cause a multiple pathological effects. Vast results of medical plants and the remarkable contribution on humans has been observed since centuries. The current study has showed the antioxidant, lipoxygenase, ACE inhibition, urease activity and reducing ability in vitro on linkus formulation, including lozenges and syrup. The anti-oxidant activities was assessed by scavenging ability of the linkus on free radical (DPPH; C18H12N5O6) 2,2’-diphenyl-1-picryl hydrazyl. For lipoxygenase measurements, purified lipoxygenase with lionoleic acid as substrate was used for the activity, however for lipoxygenase activity the thiocholine and diothiobisnitrobenzoic acid (DTNB) was used. Indophenol method has been used for determination of urease activity, however the reducing ability has been assessed by the conversion of ferric into ferrous state. In vitro results of linkus syrup as compared to standard showed good anti-oxidant and reducing ability. Moderate activity of urease, lipoxygenase and ACE inhibition were observed with comparison of standard. These activities of polyherbal formulation might be helpful for reducing cough and related symptoms.

Key words: Antioxidant activity, reducing ability, urease activity, lipoxygenase and ACE inhibition, linkus.