African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2211

Full Length Research Paper

Therapeutic potential of Annona senegalensis Pers (Annonaceae), a plant used in traditional medicine for the treatment of epilepsy in Burkina Faso

Almamy Konate
  • Almamy Konate
  • Inserm, UMR_S 1072, Marseille, France.
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Franck Dubruc
  • Franck Dubruc
  • Inserm, UMR_S 1072, Marseille, France.
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Richard W. Sawadogo
  • Richard W. Sawadogo
  • Département Médecine-Pharmacopée Traditionnelle et Pharmacie (MEPHATRA/PH), Institut de Recherche en Science de la Santé (IRSS), Ouagadougou, Burkina Faso.
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Lotfi Ferhat
  • Lotfi Ferhat
  • Aix-Marseille Université, Marseille, France.
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Norah Boumedine
  • Norah Boumedine
  • Inserm, UMR_S 1072, Marseille, France.
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Valérie Mahiou
  • Valérie Mahiou
  • Aix-Marseille Université, Marseille, France.
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Evelyne Ollivier
  • Evelyne Ollivier
  • Aix-Marseille Université, Marseille, France.
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Pierre I. Guissou
  • Pierre I. Guissou
  • Laboratoire du Développement du Médicament/Ecole Doctorale de la Science de la Santé, Université Joseph Ki-Zerbo, Ouagadougou, Burkina Faso.
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Olivier Caillard
  • Olivier Caillard
  • Inserm, UMR_S 1072, Marseille, France.
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  •  Received: 21 October 2020
  •  Accepted: 31 December 2020
  •  Published: 31 January 2021

Abstract

Annona senegalensis Pers. is a plant used in Burkina folkloric medicine for the treatment of epilepsy. The stem-bark, roots and leaves of A. senegalensis are used for an array of human disorders, including epilepsy. Previous studies have demonstrated some genuine effects of A. senegalensis fractions in vivo models of temporal lobe epilepsy. In the present work, we have compared the pharmacological profile of the most promising A. senegalensis fraction obtained up-to-date with anticonvulsant drugs targeting voltage-activated channels namely Carbamazepine (Na+ channels) and Retigabine (KCNQ Channels). In an in vitro model of temporal lobe epilepsy, we could observe a dose-dependent reduction of electrically evoked epileptiform bursts in the CA1 region of the rat hippocampus when applying carbamazepine, retigabine or the F4 fraction of A. senegalensis. The intrinsic excitability of CA1 pyramidal neurons was dose-dependently reduced by A. senegalensis. Since A. senegalensis effects could not anymore be observed when endogenous voltage-dependent sodium channels were blocked and intrinsic excitability restored with a dynamic clamp amplifier, it was concluded that A. senegalensis contains molecules that target voltage-dependent sodium channels.

Key words: Hippocampus, epilepsy, Annona senegalensis, Burkina Faso plant extracts, sodium channel, KCNQ channel, M-current, retigabine, carbamazepine.

Abbreviation

PTX, Picrotoxin; Vm, membrane potential.