African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2162

Full Length Research Paper

An optimal establishment of an acute hyperglycemia zebrafish model

Eunji Shin1#, Bin Na Hong1,2# and Tong Ho Kang1*
1College of Life Sciences, Kyung Hee University, Gyeonggi, Republic of Korea. 2Department of Audiology, Nambu University, Gwangju, Republic of Korea.
Email: [email protected]

  •  Accepted: 19 October 2012
  •  Published: 15 November 2012

Abstract

Recently, zebrafish has shown the potential to become an important in vivo model for diabetes-related research. In this study, we performed a validation study for the establishment of optimal hyperglycemia involving the following factors: alloxan concentrations of 100, 200, 300, and 400 mg/100 ml; exposure times of 30, 60, 120, and 180 min to an alloxan solution and water glucose solution; and water glucose solution concentrations of 1, 2, and 3%. The results have shown that exposure to water glucose solution following alloxan treatment might increase blood glucose level in zebrafish in a dose- and time-dependent manner. The appropriate hyperglycemia zebrafish model was induced in 300 mg/100 ml alloxan solution for 30 min, 1% water glucose solution for 30 min, and water for 1 h. We suggest that our zebrafish model could be an alternative hyperglycemia animal model in the future.

 

Key words: Zebrafish, hyperglycemia, alloxan, blood glucose.

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