African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2148

Full Length Research Paper

Effects of advanced glycation end products on expressions of EMMPRIN and MMP-2 in mouse osteoblasts

Rongfeng Dai1,2, Li Wang1, Hui Jin1 and Zilin Sun1*
1Medical College, Southeast University, Nanjing 210009, China. 2Department of Endocrinology, No.3 People’s Hospital, Changzhou 213001, China.
Email: [email protected]

  • Article Number - DF68B4029511
  • Vol.4(7), pp. 453-464, July 2010
  •  Accepted: 01 July 2010
  •  Published: 31 July 2010

Abstract

This study investigated the effects of advanced glycation end products (AGEs) on expressions of extracellular matrix metalloproteinase inducer (EMMPRIN) and matrix metalloproteinase-2 (MMP-2) in mouse osteoblasts. MC3T3-E1 cells were incubated with AGEs of different concentrations (50, 100, 200 and 400 mg/L) for various durations (12, 24 and 48 h). The expressions of EMMPRIN mRNA and protein as well as MMP-2 expression and activity were detected. Cells were treated with aminoguanidine (AG) of 0, 100, 200 and 400 µmol/L) plus 200 mg/L AGEs for 24 h, then expressions of EMMPRIN mRNA and protein were measured. Cells were treated with EMMPRIN antibody (5 μg/ml), and MMP-2 activity was determined with zymography. After AGEs treatment, the expressions of EMMPRIN mRNA and protein as well as MMP-2 expression and activity were significantly increased in a time and concentration dependent manner; AGEs plus AG of different concentrations could markedly decrease the expressions of EMMPRIN mRNA and protein when compared with controls in a concentration dependent manner; administration of EMMPRIN antibody dramatically suppressed the activity of MMP-2. AGEs could increase the expressions of EMMPRIN and MMP-2 and elevate MMP-2 activity; AG could suppress the increased expression of EMMPRIN induced by AGEs; EMMPRIN antibody conferred suppressive effects on the expression of MMP-2 induced by AGEs. Therefore, AGEs may be involved in the pathogenesis of osteoporosis via increasing the expression of EMMPRIN and MMP-2 and promoting MMP-2 activity. 

 

Key words: Advanced glycation end products, osteoblast, extracellular matrix metalloproteinase inducer, matrix metalloproteinase -2, osteoporosis.

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