African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2191

Full Length Research Paper

Effects of supplementing qi and activating blood circulation herbs on expressions of basic fibroblast growth factor and platelet endothelial cell adhesion molecule-1 in ischemic myocardium of rats with acute myocardial infarction

Shuwen Guo1*, Chenglong Zheng2, Peipei Huang1, Lu Zhang1, Panchu Yang3, Jiufeng Zhang1, Jianyu Zhou1, Kun Huang1, Dandan Yang1 and Qin Sun1
1Beijing University of Chinese Medicine, 11 Bei San Huan Dong Lu, ChaoYang District, Beijing 100029, P. R. China. 2Beijing Gulou Hospital of Tradtional Chinese Medicine,13 Dou Fu Chi Hutong, Dongcheng District,  Beijing 10009, P. R. China. 3Comprehensive Section, Liangxiang Hospital, Afflicated Hospital of Yanjing Medical College of Capital University of Medical Science, No. 45,Gongchen North street, Liangxiang Country, Fangshan District, Beijing 102401, China.
Email: [email protected]

  •  Accepted: 24 September 2012
  •  Published: 31 October 2012

Abstract

The objective of this study was to investigate the effects of supplementing qi and activating blood circulation herbs on the expressions of basic fibroblast growth factor (bFGF) and platelet endothelial cell adhesion molecule (PECAM-1) in ischemic myocardium of rats with acute myocardial infarction (AMI). Acute myocardial infarction (AMI control) of male Sprague-Dawley rats was established by ligating left anterior descending coronary artery. Rats with AMI were randomly divided into 6 groups, including AMI control, activating blood circulation herbs (blood group), supplementing qi herbs (qi group), supplementing qi herbs/activating blood circulation herbs 1:2 (1:2 group), supplementing qi herbs/activating blood circulation herbs 2:1 (2:1 group) and Tongxinluo capsule groups (TXL group), and a sham operation group was set up as negative control group. The herbs were administrated on the second day after myocardial infarction with a therapeutic course of 4 weeks, and the bFGF and PECAM-1 expressions in the myocardial area were tested by immune histochemical methods at 1, 2 and 4 weeks. The supplementing qi and activating blood circulation herbs can promote the expression of bFGF and PECAM-1 in myocardial cells, thus promoting the formation of microvasculature in myocardial ischemia and improving blood supply to the ischemic myocardium. Supplementing qi and activating blood circulation herbs increase the expression of angiogenic factors; this may be their mechanism of angiogenesis.

 

Key words: Myocardial ischemia, supplementing qi and activating blood circulation herbs, basic fibroblast growth factor, platelet endothelial cell adhesion molecule, left ventricular mass index (LVMI).