African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2220

Full Length Research Paper

Induction of apoptosis by Rhizoma Paridis saponins in MCF-7 human breast cancer cells

Chuan Lu1†, ChunJiang Li1,2†, Dongmei Wu1, JingMei Lu1*, Fan Tu1, Lijuan Wang3
1School of Life Sciences, Northeast Normal University, Renmin Street, Changchun, Jilin Province 130024, P. R. China. 2College of Basic Medicine, Jiamusi University, 148 Xuefu Street, Jiamusi, Heilongjiang Province 154007, P. R. China. 3Department of Ophthalmology, Eye Hospital, 151 East Street, Harbin, Heilongjiang Province 150001, P. R. China.
Email: [email protected]

  •  Accepted: 11 July 2011
  •  Published: 31 August 2011

Abstract

This study investigates the relationship between the induction of MCF-7 human breast cancer cell apoptosis by Rhizoma Paridis saponins (RPS) and the mitochondrial apoptotic pathway. We treated MCF-7 cells with RPS at various concentrations and examined the inhibitory effect of RPS on the proliferation of MCF-7 cells using the 4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, the change in mitochondrial membrane potential (MMP) using JC-1 staining, and the expression levels of related proteins using western blot. The results showed that the inhibitory effect of RPS against the growth of MCF-7 human breast cancer cells might be related to the apoptosis induced caspase family and the caspase-3-dependent pathway. The results suggest that RPS has the potential to be a valuable anticancer agent.

 

Key words: Rhizoma paridis saponins, MCF-7 cells, apoptosis, mitochondria

Abbreviation

RPS, Rhizoma Paridis saponins; PARP, poly ADP-ribose polymerase;FBS, fetal bovine serum; MMP, mitochondrial membrane potential; MTT, 4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.