Abstract

The present research study was designed to characterize the physiochemical, minerals, phytochemicals, antimicrobial activities and FTIR spectra analysis of various extracts ofHippophae rhamnoides L. leaves. The physiochemical and mineral content of H. rhamnoides L. leaves revealed that fiber was 18.0 ± 2.64%, protein was 10.45 ± 0.88% and carbohydrate value calculated was found to be 68.75 ± 1%. Sodium was 3,000 ± 1 ppm, calcium was 7,800 ± 1 ppm and potassium was 6,200 ± 2 ppm, referred to as a high concentration while qualitative phytochemicals investigation showed that tannins, phenols and flavonoids were present in large quantity. The highest antibacterial zone of inhibition was observed in aqueous and methanolic extracts against Staphylococcus aureus and Vibrio cholerae (21 ± 1 mm), and the lowest zone of inhibition measured 07 ± 0 mm against V. cholerae (ethyl acetate extracts). The antibacterial minimum inhibitory concentration values of extracts were determined, ranging between 40 to 120 mg/ml, and minimum bactericidal concentration values of the extracts ranged between 50 and 135 mg/ml. The highest antifungal zone of inhibition was calculated against Alternaria alternata (18 ± 0 mm) followed by Aspergillus parasiticus (17 ± 1 mm) of methanolic extract, while A. alternata (07 ± 1 mm) and Penicillium digitatum (07 ± 0 mm) were the least, found in chloroform and aqueous extract, respectively. Further assessment of antifungal minimum inhibitory concentration and minimum fungicidal concentration ranged between 40 to 135 and 50 to 180 mg/ml, respectively. The Fourier transform infrared (FTIR) spectra of all extracts revealed the presence of different functional groups ranging from hydroxyl (OH) stretching for hydroxyl group, alkanes (C-H), alkenes (C=C), aromatic rings (C=O), carboxylic (C=O) and amides (aromatic).

Key words: Seabuckthorn leaves, antibacterial, antifungal, secondary metabolites, Hippophae rhamnoides L. leaves, functional groups.