African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2283

Full Length Research Paper

Synthesis of organic compounds using yellow yam (Dioscorea praehensilis) tyrosinase as catalyst

Olutosin Samuel Ilesanmi
  • Olutosin Samuel Ilesanmi
  • Department of Chemical Sciences, Achievers University, Owo, Ondo State, Nigeria.
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Omowumi Funke Adedugbe
  • Omowumi Funke Adedugbe
  • Department of Chemical Sciences, Achievers University, Owo, Ondo State, Nigeria.
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David Adeniran Oyegoke
  • David Adeniran Oyegoke
  • Department of Chemical Sciences, Achievers University, Owo, Ondo State, Nigeria.
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Victory Ayo Olagunju
  • Victory Ayo Olagunju
  • Department of Chemistry and Biochemistry, Miami University, Oxford, Ohio, United States.
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Isaac Olusanjo Adewale
  • Isaac Olusanjo Adewale
  • Department of Biochemistry and Molecular Biology, Obafemi Awolowo University, Ile-Ife, Nigeria.
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  •  Received: 19 May 2023
  •  Accepted: 26 June 2023
  •  Published: 31 July 2023

Abstract

The authors report the synthesis of some bioactive organic compounds which could be of pharmacological interest using Dioscorea praehensilis tyrosinase immobilized on calcium-alginate beads. The enzymatic synthesis of these compounds involved pyruvic acid as a source of carbonyl groups, ammonia and catechol. Catechol was used as substrate and a parent compound that supplied aromatic ring carbons for the reactions. The structures of novel compounds synthesized were deduced using UV, IR and NMR spectroscopic techniques. Structural elucidation revealed that a novel lactone (3-hydroxyl-3-methylbenzo[b][1,4]-lactone) was synthesized in the presence of pyruvic acid and catechol only. When ammonia solution was introduced into the reaction system, there was conversion of the blue-colored lactone compound into a red-colored product. The structure was elucidated as 2-(2-hydroxyphenoxy)-2-aminopropanoic acid. Whereas, in the presence of ammonia and catechol only, a novel secondary amine (1,6-dihydroxy-7-aza,bicyclo[4.1.0]-2,4-heptadiene) was synthesized. Though the synthesized compounds possess functional groups, the specific pharmacological or biotechnological applications are yet to be investigated and established.

Key words: Yam Tyrosinase, catechol, novel lactone, novel amino acid, novel secondary amine.