African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2191

Full Length Research Paper

Effects of PEGylation on the Lowry method for the content determination of trichosanthin

Yan Ping Li1, Li Hu Zhang1, Li Ying Jiang1, Long Fu Rong2, Xian Yu Du2,3 and Li Hua Song2,3*
1Yancheng Health Vocational and Technical College, Jiangsu Province, P. R. China. 2Anke Biotechnology (Group) Co., Ltd., Anhui Province, P. R. China. 3Anhui Biological Institute, Anhui Province, P. R. China.
Email: [email protected]

  •  Accepted: 20 September 2012
  •  Published: 31 October 2012

Abstract

The current study was designed to establish a method for examining whether the content of PEG-TCS could be determined by the Lowry method. The research applicability of the Lowry method on the determination of PEGylated trichosanthin (PEG-TCS) protein content was proposed for the first time. Procedures were designed to determine whether residual PEG interfered with the result of protein content determination, PEG (≤20 µg/ml) interference was not apparent. The interference of a PEG-modified group was analyzed by ultra violet spectrophotometric colorimetry, and the differences in the rates were 5.0 and 6.2%. The linearity, precision, and recovery were determined. There is good linear relationship from 30 to 70 μg/ml PEG-TCS. The experiment results provided sufficient data to establish a quality control method for the determination of PEG-TCS content, and they were conducive to the determination method establishment of other PEGylated proteins for non clinical experiment application.

 

Key words: PEGylated trichosanthin, Lowry method, trichosanthin, methodology validation.