African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2208

Article in Press

RP-HPLC method development and validation for the simultaneous estimation of atorvastatin, simvastatin and rosuvastatin in bulk and pharmaceutical dosage form

Bhuvnesh Kumar Singh*, Neelanchal Trivedi, Abhishek Bharadwaj and K. K. Jha

  •  Received: 05 March 2018
  •  Accepted: 25 April 2018
This research was designed to develop and validate a precise, simple, rapid, reliable, more accurate, as well as least time consuming High-Performance Liquid Chromatography (HPLC) technique for simultaneous and individual estimation of atorvastatin, rosuvastatin and simvastatin in whole and in various formulations. This separation was attained chromatographically using blend of methanol and trifluroacetic acid (0.1%) in water (60:25) as mobile phase, with a hypersil ODS C18 (250 mm × 4.6 mm, i.d. 2.5 µm) column with flow rate at 1.0 mL/min and the eluents were monitored at 235 nm. A significant outcome in form of linearity was shown by all three drugs, that is, atorvastatin within the concentration range from 0.14 to 0.24 mg/mL, while simvastatin 0.1 to 0.14 mg/mL, whereas rosuvastatin showed the same in concentration range from 0.12 to 0.24 mg/ml. The accuracy was found to be decent with recovery values ranged from 99.21 to 100.40%, 99.87 to 100.39%, and 98.84 to 100.66%, respectively for atorvastatin, simvastatin and rosuvastatin. Thus, the method acclaimed to be simple, linear, selective, precise, sensitive and accurate, hence can be employed successfully for routinely determination of control of quality for formulations encompassing both the drugs in pharmaceutical industries.

Keywords: Atorvastatin, simvastatin, rosuvastatin, validation