Amino group of thiazole moiety (26) was treated with various aromatic aldehydes its gave corresponding Schiff's bases (27-36). These Schiff's bases were reacted with benzyl in the presence of ammonium acetate (NH4OAc) to find corresponding novel 2-substituted aminothiazoles (37-46). Structures of all the newly synthesized compounds were confirmed by IR, 1H NMR, mass spectral data and elemental analysis. The synthesized compounds were evaluated for the antibacterial and short-term anticancer activity. All the synthesized 2-substituted aminothiazoles was shown good antibacterial activity against gram negative bacterial strain Escherichia coli. The synthesized 2-substituted aminothiazoles derivative possessed significant cytotoxic activity against DaltonêžŒs Lymphoma Ascites (DLA) and EhrlichêžŒs Ascites Carcinoma (EAC) cell lines.