African Journal of
Plant Science

  • Abbreviation: Afr. J. Plant Sci.
  • Language: English
  • ISSN: 1996-0824
  • DOI: 10.5897/AJPS
  • Start Year: 2007
  • Published Articles: 745

Full Length Research Paper

Molecular characterization of garlic (Allium sativum L.) using SSR markers in Ethiopia

Kalkidan Tesfu
  • Kalkidan Tesfu
  • Ethiopian Institute of Agricultural Research, National Agricultural Biotechnology Research Center, P. O. Box 249, Holeta, Ethiopia.
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Nitsuh Aschale
  • Nitsuh Aschale
  • Ethiopian Institute of Agricultural Research, National Agricultural Biotechnology Research Center, P. O. Box 249, Holeta, Ethiopia.
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Emebet Regassa
  • Emebet Regassa
  • Ethiopian Institute of Agricultural Research, National Agricultural Biotechnology Research Center, P. O. Box 249, Holeta, Ethiopia.
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Helen Gebremdhen
  • Helen Gebremdhen
  • Ethiopian Institute of Agricultural Research, National Agricultural Biotechnology Research Center, P. O. Box 249, Holeta, Ethiopia.
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Obsi Desalegn
  • Obsi Desalegn
  • Ethiopian Institute of Agricultural Research, National Agricultural Biotechnology Research Center, P. O. Box 249, Holeta, Ethiopia.
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Demilew Deres
  • Demilew Deres
  • Ethiopian Institute of Agricultural Research, National Agricultural Biotechnology Research Center, P. O. Box 249, Holeta, Ethiopia.
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Sara Gebremeskel
  • Sara Gebremeskel
  • Ethiopian Institute of Agricultural Research, National Agricultural Biotechnology Research Center, P. O. Box 249, Holeta, Ethiopia.
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  •  Received: 06 May 2020
  •  Accepted: 21 January 2021
  •  Published: 28 February 2021

Abstract

Garlic is one of the most cultivated and medicinal horticultural crops in the world. Its production and productivity is decreased due to different constraints. Knowing the genetic distance will give information to tackle the constraints. This study characterized genetic diversity of garlic germplasm by analysis of eleven Simple Sequence Repeats (SSR) loci in 115 garlic accession. Genomic DNA was extracted from fresh leaf tip using modified Diversity Array Technology (DArT) protocol. Touch down polymerase chain reaction (PCR) was also used to get optimal annealing temperature for each Simple Sequence Repeats (SSR) primers. A number of monomorphic and few polymorphic bands were obtained from eleven SSR markers. The gel pictures show monomorphic bands between genotypes. However, some markers: ASM 080, ASA 20, ASA23, and GB-ASM 053 generated polymorphic bands. Dendrogram of genetic distances amongst all tested genotypes showed three distinct major clusters, and six sub-clusters. This study will guide decision making on introduction of germplasm for enhancing genetic diversity or creation of new variations to improve and widen the genetic base of garlic.

 

Key words: Garlic, genetic variation, polymorphism.