African Journal of
Plant Science

  • Abbreviation: Afr. J. Plant Sci.
  • Language: English
  • ISSN: 1996-0824
  • DOI: 10.5897/AJPS
  • Start Year: 2007
  • Published Articles: 718

Full Length Research Paper

Molecular analysis and phenotype characterization of the progeny of two antisense potato plants

Olawole O. Obembe1,2*, Evert Jacobsen1 and Richard G. F. Visser1
  1Laboratory of Plant Breeding, Wageningen University, Box 386 6700AJ Wageningen, The Netherlands 2Present address: Department of Biological sciences Covenant University, P. M. B 1023 Ota, Ogun State, Nigeria.
Email: [email protected]

  •  Accepted: 16 June 2008
  •  Published: 30 June 2008

Abstract

 

Two transgenic potato lines csr2-1 and csr4-8, containing two different antisense constructs, csr2 and csr4, respectively, were crossed to investigate the possibility of achieving double transformants with combined effects of the two antisense transgenes on plant phenotypes and cellulose deposition. Molecular analysis revealed an expected segregation ratio of 1:1:1:1 of the four classes. Phenotype characterization revealed that offspring containing either one or both transgenes produced more tubers than the control plants but individual tubers were mostly smaller and had lesser weight than the control tubers.

 

Key words: Antisense technology, double transformants, genetic crossing, potato, segregation analysis.