Abstract

This research investigated pseudobulb morphological development process of Cremastra appendiculata (D. Don) Makino by paraffin section and micro-observation combining morphological observation method, and analyzed correlation between pseudobulb morphogenesis and its part of biochemical compositions to provide clues for revealing the inhibition mechanism of germination of elder pseudobulbs by the newborn one. The results showed that pseudobulb morphogenesis of C. appendiculata can be divided into six stages: Leaf bud dormant stage, leaf bud sprouting stage, elongation growth stage of leaf bud and rhizomes, pseudobulb initial formation stage, pseudobulb swelling stage and pseudobulb full development stage. There was a close relationship between the changes of the soluble sugar and protein contents both in the newborn and elder pseudobulb development soluble sugar contents were the maximum in leaf bud dormant stage. And in annual, biennial and triennial pseudobulbs, soluble sugar contents were 23.94, 34.21 and 39.02 mg·g^-1 respectively. However, soluble protein contents were the minimum in annual, biennial and triennial pseudobulbs, which were 1.30, 1.43 and 1.58 mg·g^-1, respectively. With the leaf buds germinating, soluble sugar and protein contents were presenting the change trend of decline firstly, rising secondly, and then declining.

Key words: Cremastra appendiculata (D. Don) Makino, pseudobulb, morphogenesis, biochemical component.