African Journal of
Plant Science

  • Abbreviation: Afr. J. Plant Sci.
  • Language: English
  • ISSN: 1996-0824
  • DOI: 10.5897/AJPS
  • Start Year: 2007
  • Published Articles: 757

Full Length Research Paper

The correlation between pseudobulb morphogenesis and main biochemical components of Cremastra appendiculata (D. Don) Makino

Xiao-Feng Gao
  • Xiao-Feng Gao
  • School of Life Sciences, Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Guizhou University, Guiyang, 550025 Guizhou, People’s Republic of China.
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Xiang Lv
  • Xiang Lv
  • School of Life Sciences, Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Guizhou University, Guiyang, 550025 Guizhou, People’s Republic of China.
  • Google Scholar
Xiao-Lan Li
  • Xiao-Lan Li
  • School of Life Sciences, Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Guizhou University, Guiyang, 550025 Guizhou, People’s Republic of China.
  • Google Scholar
Ming-Sheng Zhang
  • Ming-Sheng Zhang
  • School of Life Sciences, Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Guizhou University, Guiyang, 550025 Guizhou, People’s Republic of China.
  • Google Scholar
Yan-Qiu Wu
  • Yan-Qiu Wu
  • School of Life Sciences, Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Guizhou University, Guiyang, 550025 Guizhou, People’s Republic of China.
  • Google Scholar
Wang-Zhong Wang
  • Wang-Zhong Wang
  • School of Life Sciences, Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Guizhou University, Guiyang, 550025 Guizhou, People’s Republic of China.
  • Google Scholar


  •  Received: 22 March 2016
  •  Accepted: 01 May 2016
  •  Published: 31 May 2016

Abstract

This research investigated pseudobulb morphological development process of Cremastra appendiculata (D. Don) Makino by paraffin section and micro-observation combining morphological observation method, and analyzed correlation between pseudobulb morphogenesis and its part of biochemical compositions to provide clues for revealing the inhibition mechanism of germination of elder pseudobulbs by the newborn one. The results showed that pseudobulb morphogenesis of C. appendiculata can be divided into six stages: Leaf bud dormant stage, leaf bud sprouting stage, elongation growth stage of leaf bud and rhizomes, pseudobulb initial formation stage, pseudobulb swelling stage and pseudobulb full development stage. There was a close relationship between the changes of the soluble sugar and protein contents both in the newborn and elder pseudobulb development soluble sugar contents were the maximum in leaf bud dormant stage. And in annual, biennial and triennial pseudobulbs, soluble sugar contents were 23.94, 34.21 and 39.02 mg·g-1 respectively. However, soluble protein contents were the minimum in annual, biennial and triennial pseudobulbs, which were 1.30, 1.43 and 1.58 mg·g-1, respectively. With the leaf buds germinating, soluble sugar and protein contents were presenting the change trend of decline firstly, rising secondly, and then declining.

Key words: Cremastra appendiculata (D. Don) Makino, pseudobulb, morphogenesis, biochemical component.