International Journal of
Biotechnology and Molecular Biology Research

  • Abbreviation: Int. J. Biotechnol. Mol. Biol. Res.
  • Language: English
  • ISSN: 2141-2154
  • DOI: 10.5897/IJBMBR
  • Start Year: 2010
  • Published Articles: 100

Full Length Research Paper

Genetic polymorphism of growth hormone locus and its association with bodyweight in Grati dairy cows

Sucik Maylinda
Faculty of Animal Husbandry, Brawijaya University, Malang – Indonesia
Email: [email protected]

  •  Accepted: 29 June 2011
  •  Published: 31 July 2011


The aim of this research was to study genetic polymorphism in growth hormone (GH) locus and its association with body weight of 43 Grati dairy cows ranging from 2 to 4 years old. Polymorphism of GH locus was ascribed using  a PCR-RFLP method involving restricted enzyme Msp1 on 2% agarose gel. All data were subjeced to statistical analysis based on one way classification model using a statistical software package of Genstat 200 version 2. The results showed that frequencies of normal allel (Msp1+) and mutant allel (Msp-) were 0.34 and 0.66 respectively with 0.22 polymorphism was found in this locus. There was significant relationship between Msp1+/Msp1+, Msp1+/Msp1- and Msp1-/Msp1- genotypes and body weight (P < 0.05) but Msp1+/Msp1+ and Msp1+/Msp1- genotypes had a stronger correlation to the higher body weight than Msp1-/Msp1- genotype.

Key words: Grati cows, polymorphism, growth hormone, body weight.