International Journal of
Biotechnology and Molecular Biology Research

  • Abbreviation: Int. J. Biotechnol. Mol. Biol. Res.
  • Language: English
  • ISSN: 2141-2154
  • DOI: 10.5897/IJBMBR
  • Start Year: 2010
  • Published Articles: 100

Full Length Research Paper

Genetic polymorphism of growth hormone locus and its association with bodyweight in Grati dairy cows

Sucik Maylinda
Faculty of Animal Husbandry, Brawijaya University, Malang – Indonesia
Email: [email protected]

  •  Accepted: 29 June 2011
  •  Published: 31 July 2011

Abstract

The aim of this research was to study genetic polymorphism in growth hormone (GH) locus and its association with body weight of 43 Grati dairy cows ranging from 2 to 4 years old. Polymorphism of GH locus was ascribed using  a PCR-RFLP method involving restricted enzyme Msp1 on 2% agarose gel. All data were subjeced to statistical analysis based on one way classification model using a statistical software package of Genstat 200 version 2. The results showed that frequencies of normal allel (Msp1+) and mutant allel (Msp-) were 0.34 and 0.66 respectively with 0.22 polymorphism was found in this locus. There was significant relationship between Msp1+/Msp1+, Msp1+/Msp1- and Msp1-/Msp1- genotypes and body weight (P < 0.05) but Msp1+/Msp1+ and Msp1+/Msp1- genotypes had a stronger correlation to the higher body weight than Msp1-/Msp1- genotype.

Key words: Grati cows, polymorphism, growth hormone, body weight.