International Journal of
Genetics and Molecular Biology

  • Abbreviation: Int. J. Genet. Mol. Biol.
  • Language: English
  • ISSN: 2006-9863
  • DOI: 10.5897/IJGMB
  • Start Year: 2009
  • Published Articles: 115

Full Length Research Paper

Investigation of polymorphisms of exon 1 region of OsHKT1;5 gene in high yielding rice

Nguyen Thi Pha
  • Nguyen Thi Pha
  • Biotechnology Research and Development Institute, Cantho University, Cantho, Vietnam.
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Nguyen Thi Phung
  • Nguyen Thi Phung
  • Biotechnology Research and Development Institute, Cantho University, Cantho, Vietnam.
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Nguyen Thi Ngoc Truc
  • Nguyen Thi Ngoc Truc
  • Biotechnology Research and Development Institute, Cantho University, Cantho, Vietnam.
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Do Tan Khang
  • Do Tan Khang
  • Biotechnology Research and Development Institute, Cantho University, Cantho, Vietnam.
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Tran Dinh Gioi
  • Tran Dinh Gioi
  • Genetics and Plant Breeding Division, Cuu Long Delta Rice Research Institute, Thoilai, Cantho, Vietnam.
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  •  Received: 24 March 2019
  •  Accepted: 16 May 2019
  •  Published: 30 June 2019

Abstract

This study was carried out to analyze the polymorphic region of exon 1 of OsHKT1;5 of 22 high yielding rice varieties that are salt tolerant. The result of the phenotypic screening for salinity stress was done using the standard protocol of the International Rice Research Institute which recorded that all rice varieties developed very well under control condition, 85.38% were quite tolerant to salinity treatment of 4‰, 68.18% were moderately tolerant to salinity treatment of 8‰ and 36.36% were moderately tolerant to salinity treatment of 10‰, similar to Pokalli. The result of sequencing exon 1 region of OsHKT1;5 gene recorded five Single Nucleotide Polymorphisms (SNP) markers. Five nucleotide substitutions in coding sequence of OsHKT1;5 were found at the positions: 382, ​​418, 484, 551 and 994. All five non-synonymous nucleotide substitutions caused changes in amino acids (D51N, P63A, V86I, R107H and H255D).

 

Key words: High yielding rice, OsHKT1;5, single nucleotide polymorphisms (SNP) makers.