International Journal of
Genetics and Molecular Biology

  • Abbreviation: Int. J. Genet. Mol. Biol.
  • Language: English
  • ISSN: 2006-9863
  • DOI: 10.5897/IJGMB
  • Start Year: 2009
  • Published Articles: 126

Full Length Research Paper

Mapping of fourteen high-risk human papillomavirus genotypes by molecular detection in sexually active women in the West African sub-region

Théodora Mahoukèdè Zohoncon
  • Théodora Mahoukèdè Zohoncon
  • Laboratory of Molecular Biology and Genetics (LABIOGENE), University of Ouagadougou, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Wendkuuni Florencia Djigma
  • Wendkuuni Florencia Djigma
  • Laboratory of Molecular Biology and Genetics (LABIOGENE), University of Ouagadougou, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Abdoul Karim Ouattara
  • Abdoul Karim Ouattara
  • Laboratory of Molecular Biology and Genetics (LABIOGENE), University of Ouagadougou, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Ina M.A Traore
  • Ina M.A Traore
  • Laboratory of Molecular Biology and Genetics (LABIOGENE), University of Ouagadougou, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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R. Alice Ouedraogo
  • R. Alice Ouedraogo
  • Laboratory of Molecular Biology and Genetics (LABIOGENE), University of Ouagadougou, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Esther M.A Traoré
  • Esther M.A Traoré
  • Laboratory of Molecular Biology and Genetics (LABIOGENE), University of Ouagadougou, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Prosper Bado
  • Prosper Bado
  • Laboratory of Molecular Biology and Genetics (LABIOGENE), University of Ouagadougou, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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T. Clarisse Ouedraogo
  • T. Clarisse Ouedraogo
  • Laboratory of Molecular Biology and Genetics (LABIOGENE), University of Ouagadougou, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Birama Diarra
  • Birama Diarra
  • Laboratory of Molecular Biology and Genetics (LABIOGENE), University of Ouagadougou, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Maimouna Ilboudo
  • Maimouna Ilboudo
  • Laboratory of Molecular Biology and Genetics (LABIOGENE), University of Ouagadougou, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Callinice D. Capo-chichi
  • Callinice D. Capo-chichi
  • University of Abomey-Calavi, Benin, 01 BP 526 Cotonou, Benin.
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Dorcas Obiri-Yeboah
  • Dorcas Obiri-Yeboah
  • University of Cape Coast, School of Medical Sciences, Department of Microbiology and Immunology, University Post Office, Tel. +233 332 132 440; Fax: +233 332 132 484, Ghana.
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Simplice Damintoti Karou
  • Simplice Damintoti Karou
  • Pietro Annigoni Biomolecular Research Centre (CERBA), 01 BP 364 Ouagadougou 01, Burkina Faso.
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Mady Nayama
  • Mady Nayama
  • University Abdoul Moumouni, Niamey, BP 237 – Niamey, Niger.
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Apollinaire Horo
  • Apollinaire Horo
  • University Félix Houphouët Boigny, UFR Des Sciences Médicales D'Abidjan,01 BPV 34 Abidjan, Ivory Coast.
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Kouame Privat Kouakou
  • Kouame Privat Kouakou
  • University of Bouaké, Côte d'Ivoire, CHU de Bouaké, BP V 18 01, Bouaké Ivory Coast.
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Moutawakilou Gomina
  • Moutawakilou Gomina
  • University of Parakou, Benin, BP 123 Parakou, Benin.
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Souleymane Ouattara
  • Souleymane Ouattara
  • University Polytechnique of Bobo, 01 BP 1091, Bobo-Dioulasso - Burkina Faso.
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Ambaliou Sanni
  • Ambaliou Sanni
  • University of Abomey-Calavi, Benin, 01 BP 526 Cotonou, Benin
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Akpona Simon
  • Akpona Simon
  • University of Parakou, Benin, BP 123 Parakou, Benin.
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Charlemagne Ouedraogo
  • Charlemagne Ouedraogo
  • Pietro Annigoni Biomolecular Research Centre (CERBA), 01 BP 364 Ouagadougou 01, Burkina Faso.
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Jacques Simpore
  • Jacques Simpore
  • Laboratory of Molecular Biology and Genetics (LABIOGENE), University of Ouagadougou, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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  •  Received: 26 December 2019
  •  Accepted: 29 January 2020
  •  Published: 29 February 2020

Abstract

The aim of this study was to determine the distribution of high-risk human papillomavirus genotypes (HR-HPV) in women from the general population of five West African countries. This was a cross-sectional descriptive study, involving 2133 women from nine cities of five West African countries: Benin, Burkina Faso, Côte d'Ivoire, Niger and Togo. Women were screened for precancerous cervical lesions and HR-HPV infection. The detection of HR-HPV was done by a multiplex real-time PCR on extracted viral DNA. The average age of the women in this study was 35.06 ± 10.00 years with a range of 15 to 65 years. The overall prevalence of high-risk HPV infection among general population sample of women in five West African countries was 33.61% (717/2133). The prevalence of dysplasia was 8.81%. In decreasing order of frequency, the genotypes found were: HPV 52 followed by HPV 31, 59, 51, 66, 45, 68, 56, 56, 58, 35, 39, 18, 33 and 16. The prevalence of HPV16/18 (bivalent vaccine types) was 7.02%. This study reveals a high prevalence of HPV 52 in West Africa. The extent and diversity of HR-HPV genotypes in these West African countries deserve special attention for prevention.

 

Key words: High-risk HPV, real time PCR, genotypes, women, epidemiology, West Africa.