International Journal of
Genetics and Molecular Biology

  • Abbreviation: Int. J. Genet. Mol. Biol.
  • Language: English
  • ISSN: 2006-9863
  • DOI: 10.5897/IJGMB
  • Start Year: 2009
  • Published Articles: 126

Full Length Research Paper

Impact of glutathione S-transferase genes polymorphisms on human papillomavirus infection and precancerous lesions in West African women

Teega-Wendé Clarisse OUEDRAOGO
  • Teega-Wendé Clarisse OUEDRAOGO
  • Laboratory of Molecular Biology and Genetics (LABIOGENE), University Joseph Ki-Zerbo, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Florencia Wendkuuni DJIGMA
  • Florencia Wendkuuni DJIGMA
  • Laboratory of Molecular Biology and Genetics (LABIOGENE), University Joseph Ki-Zerbo, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Boureima IDANI
  • Boureima IDANI
  • Laboratory of Molecular Biology and Genetics (LABIOGENE), University Joseph Ki-Zerbo, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Théodora Mahoukèdè ZOHONCON
  • Théodora Mahoukèdè ZOHONCON
  • Laboratory of Molecular Biology and Genetics (LABIOGENE), University Joseph Ki-Zerbo, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Pegdwendé Abel SORGHO
  • Pegdwendé Abel SORGHO
  • Laboratory of Molecular Biology and Genetics (LABIOGENE), University Joseph Ki-Zerbo, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Prosper BADO
  • Prosper BADO
  • Laboratory of Molecular Biology and Genetics (LABIOGENE), University Joseph Ki-Zerbo, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Mah Alima Esther TRAORE
  • Mah Alima Esther TRAORE
  • Laboratory of Molecular Biology and Genetics (LABIOGENE), University Joseph Ki-Zerbo, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Abdoul Karim OUATTARA
  • Abdoul Karim OUATTARA
  • Laboratory of Molecular Biology and Genetics (LABIOGENE), University Joseph Ki-Zerbo, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Marius Ayaovi SETOR
  • Marius Ayaovi SETOR
  • Laboratory of Molecular Biology and Genetics (LABIOGENE), University Joseph Ki-Zerbo, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Shoukrat Ohuwa Toyin BELLO
  • Shoukrat Ohuwa Toyin BELLO
  • Laboratory of Molecular Biology and Genetics (LABIOGENE), University Joseph Ki-Zerbo, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Simplice Damintoti KAROU
  • Simplice Damintoti KAROU
  • Higher School of Biological and Food Techniques (ESTBA-UL), University of Lome, 01 BP 1515 Lome, Togo.
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Apollinaire HORO
  • Apollinaire HORO
  • Faculty of Medical Sciences of Abidjan, University Felix Houphouet Boigny, 01 BP V34 Abidjan, Côte d’Ivoire.
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Kouame Privat KOUAKOU
  • Kouame Privat KOUAKOU
  • Department of Medical Sciences, University of Bouake, CHU of Bouake, BP V 18 01, Bouake, Côte d’Ivoire.
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Moutawakilou GOMINA
  • Moutawakilou GOMINA
  • Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Parakou, BP: 123 Parakou Benin.
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Mady NAYAMA
  • Mady NAYAMA
  • Faculty of Health Science, University Abdoul Moumouni, BP 237 Niamey, Niger.
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D. Callinice CAPO-CHICHI
  • D. Callinice CAPO-CHICHI
  • Institute of Biomedical Sciences and Applications, Faculty of Sciences and Technology (FAST), University of Abomey-Calavi / UAC, 01 BP 526 Cotonou, Benin.
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Ambaliou SANNI
  • Ambaliou SANNI
  • Institute of Biomedical Sciences and Applications, Faculty of Sciences and Technology (FAST), University of Abomey-Calavi / UAC, 01 BP 526 Cotonou, Benin.
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Dorcas OBIRI-YEBOAH
  • Dorcas OBIRI-YEBOAH
  • Department of Microbiology and Immunology, School of Medical Sciences, University of Cape Coast, Ghana.
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Simon AKPONA
  • Simon AKPONA
  • Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Parakou, BP: 123 Parakou Benin.
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Albert Théophane YONLI
  • Albert Théophane YONLI
  • Laboratory of Molecular Biology and Genetics (LABIOGENE), University Joseph Ki-Zerbo, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Charlemagne OUEDRAOGO
  • Charlemagne OUEDRAOGO
  • UFR of Health Science, University Joseph Ki-Zerbo of Ouagadougou, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Jacques SIMPORE
  • Jacques SIMPORE
  • Laboratory of Molecular Biology and Genetics (LABIOGENE), University Joseph Ki-Zerbo, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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  •  Received: 02 March 2020
  •  Accepted: 05 June 2020
  •  Published: 31 July 2020

Abstract

Genetic polymorphisms of certain classes of glutathione S-transferase (GST), enzyme responsible for the biotransformation of drugs and xenobiotics, have been associated with risk of several cancers such as cervical cancer. The aim of this study is to investigate the impact of glutathione S-transferase M1 and T1 deletion on high-risk human papillomavirus (HR-HPV) infections and on dysplasia. A case-control study was carried out on 1069 endocervical samples from West African women including 482 HR-HPV positive and 139 patients had cervical lesions according to visual inspection with acetic acid and Lugol (VIA/VILI) screening. Deletion of the GSTM1 and GSTT1 genes was determined using conventional PCR and genotypes of HR-HPV by real-time PCR. An association with a reduced risk for HR-HPV infection was observed in Ivorian population with GSTT1-null (OR = 0.61, 95% CI = 0.40 - 0.92, p= 0.02) and GSTM1-active/GSTT1-null genotypes (OR = 0.56, 95% CI = 0.35 - 0.90, p= 0.02). In West African, women with GSTT1-null genotype had 1.72-fold higher risk for infection with HPV66 (p= 0.044) and reduced risk (OR = 0.39) for HPV35. Whereas women with GSTM1-null/GSTT1-active genotype had 2.32-fold higher risk for HPV18 infection (p= 0.042). GSTT1-null genotype was associated to cervical lesions in West African with a reduced risk (OR = 0.63, p= 0.017).  The results of the present study demonstrate that GSTT1-null could be associated with cervical lesions and HPV35 infection with reduced risk. GSTM1-null associated with GSTT1-active could play a role in increasing the risk for HPV18 infection.

 

Key words: Cervical cancer, GSTM1, GSTT1, HR-HPV, West Africa.