International Journal of
Genetics and Molecular Biology

  • Abbreviation: Int. J. Genet. Mol. Biol.
  • Language: English
  • ISSN: 2006-9863
  • DOI: 10.5897/IJGMB
  • Start Year: 2009
  • Published Articles: 131

Full Length Research Paper

Iron starvation induces expression of a putative xylanase gene in Salmonella enterica subsp. enterica serovar Enteritidis mini-transposon5 lacZ1 mutants

Kazhila C. Chinsembu* and André Faul
Department of Biological Sciences, Faculty of Science, University of Namibia, P/Bag 13301, Windhoek, Namibia.
Email: [email protected], [email protected]

  •  Accepted: 30 June 2010
  •  Published: 30 July 2010

Abstract

 

In this study, Salmonella enterica subsp. enterica serovar Enteritidis mini-transposon5 lacZ1 mutant strains induced under iron starvation were analyzed. Inverse polymerase chain reaction (IPCR) was used to isolate iron starvation-induced DNA fragments upstream of mini-Tn5 (containing a promoter-less lacZ gene) insertion in S. entericasubsp. enterica serovar Enteritidis mutants showing β-galactosidase activity during growth in Fe3+-deprived media. Out of ten mutant strains analyzed, four (Ez188, Ez477, Ez1819 and Ez2508) were induced during growth in the presence of the iron chelator, 2,2’-dipyridyl. IPCR products of Ez188 and Ez2508 were reamplified by nested PCR. Sequence analysis of the Ez188 PCR product revealed that a putative xylanase gene was induced under iron starvation conditions.

 

Key words: Salmonella mutants, iron starvation, induced, genes, putative xylanase.