In this study, Salmonella enterica subsp. enterica serovar Enteritidis mini-transposon5 lacZ1 mutant strains induced under iron starvation were analyzed. Inverse polymerase chain reaction (IPCR) was used to isolate iron starvation-induced DNA fragments upstream of mini-Tn5 (containing a promoter-less lacZ gene) insertion in S. entericasubsp. enterica serovar Enteritidis mutants showing β-galactosidase activity during growth in Fe3+-deprived media. Out of ten mutant strains analyzed, four (Ez188, Ez477, Ez1819 and Ez2508) were induced during growth in the presence of the iron chelator, 2,2’-dipyridyl. IPCR products of Ez188 and Ez2508 were reamplified by nested PCR. Sequence analysis of the Ez188 PCR product revealed that a putative xylanase gene was induced under iron starvation conditions.
Key words: Salmonella mutants, iron starvation, induced, genes, putative xylanase.
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