International Journal of
Genetics and Molecular Biology

  • Abbreviation: Int. J. Genet. Mol. Biol.
  • Language: English
  • ISSN: 2006-9863
  • DOI: 10.5897/IJGMB
  • Start Year: 2009
  • Published Articles: 115

Full Length Research Paper

The characterization of Citrus sp. from Parang Island Karimunjawa based on morphological, DNA barcoding and nutritional analysis

Hermin Pancasakti Kusumaningrum1*,
  • Hermin Pancasakti Kusumaningrum1*,
  • Department of Biology, Faculty of Sciences and Mathematic, Diponegoro University, Jl. Prof Sudharto SH. Tembalang Semarang 50275, Phone/Fax +62-02476480923, Indonesia.
  • Google Scholar
Anto Budiharjo
  • Anto Budiharjo
  • Department of Biology, Faculty of Sciences and Mathematic, Diponegoro University, Jl. Prof Sudharto SH. Tembalang Semarang 50275, Phone/Fax +62-02476480923, Indonesia.
  • Google Scholar
Agung Suprihadi
  • Agung Suprihadi
  • Department of Biology, Faculty of Sciences and Mathematic, Diponegoro University, Jl. Prof Sudharto SH. Tembalang Semarang 50275, Phone/Fax +62-02476480923, Indonesia.
  • Google Scholar
Yuriza Eshananda
  • Yuriza Eshananda
  • Department of Biology, Faculty of Sciences and Mathematic, Diponegoro University, Jl. Prof Sudharto SH. Tembalang Semarang 50275, Phone/Fax +62-02476480923, Indonesia.
  • Google Scholar
Annisa Fadillah
  • Annisa Fadillah
  • Department of Biology, Faculty of Sciences and Mathematic, Diponegoro University, Jl. Prof Sudharto SH. Tembalang Semarang 50275, Phone/Fax +62-02476480923, Indonesia.
  • Google Scholar
Dina Rahayuning Pangestuti
  • Dina Rahayuning Pangestuti
  • Faculty of Public Health, Diponegoro University, Jl. Prof Sudharto SH. Tembalang Semarang 50275, Indonesia.
  • Google Scholar


  •  Received: 21 June 2018
  •  Accepted: 04 September 2018
  •  Published: 30 September 2018

References

Abirami A, Nagarani G, Siddhuraju P (2014). In vitro antioxidant, anti-diabetic, cholinesterase and tyrosinase inhibitory potential of fresh juice from Citrus hystrix and C. maxima fruits. Food Science and Human Wellness 3:6-25.
Crossref

 

Acosta-Motos J, Ortu-o MF, Bernal-Vicente A, Diaz-Vivancos P, Sanchez-Blanco M, Hernandez JA (2017). Plant responses to salt stress: adaptive mechanisms. Agronomy Journal 7(18):1-38.

 
 

Adelina SO, Adelina E (2017). Identifikasi morfologi dan anatomi jeruk lokal (Citrus sp) di desa Doda dan desa Lempe Kecamatan Lore. Jurnal Agrotekbis (In Indonesian) 5(1):58-65.

 
 

Agisimanto D, Martasari C, Supriyanto A (2007). Perbedaan primer RAPD dan ISSR dalam identifikasi hubungan kekerabatan genetik jeruk siam (Citrus suhuniensis L. Tan) Indonesia. Jurnal Hortikultura (In Indonesian) 17(1):101-110.

 
 

Álvarez I, Wendel JF (2003). Ribosomal ITS sequences and plant phylogenetic inference. Molecular Phylogenetics and Evolution 29:417-434.
Crossref

 
 

AOAC (Association of Official Analitycal Chemists) (2005). Official method of analysis (18th Edition). Washington.DC: Association of Officiating Analytical Chemists.

 
 

Bayer RJ, Mabberley DJ, Morton C, Miller CH, Sharma IK, Pfeil BE, Rich S, Hitchcock R, Sykes S (2009). A molecular phylogeny of the orange subfamily (Rutaceae: Aurantioideae) using nine cpDNA sequences. American Journal of Botany 96(3):668-685.
Crossref

 
 

Bulley SM, Rassam M, Hoser D, Otto W, Schünemann N, Wright M, MacRae E, Gleave A, Laing W. (2009). Gene expression studies in kiwifruit and gene over-expression in Arabidopsis indicates that GDP-L-galactose guanyltransferase is a major control point of vitamin C biosynthesis. Journal of Experimental Botany 60(3):765-778.
Crossref

 
 

Chiba H, Uehara M, Wu J, Wang X, Masuyama R, Suzuki K, Kanazawa K, Ishimi, Y (2003). Hesperidin, a citrus flavonoid, inhibits bone loss and decreases serum and hepatic lipids in ovariectomized mice. Journal of Nutrition 133(6):1892-1897.
Crossref

 
 

Ciancaglini P, Santos HL, Daghastanli KRP, Thedei G (2001). Using a classical method of vitamin C quantification as a tool for discussion of its role in the body. Biochemistry and Molecular Biology Education 29(3):110-114.

 
 

Davey MW, Kenis K, Keulemans J (2006) Genetic control of fruit vitamin C contents. Plant Physiology 142(1):343-351.
Crossref

 
 

Direktorat Jenderal Holtikultura (2015). Sub Sektor Holtikultura,  (In Indonesian).

 
 

Doyle JJ, Doyle JL (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19:11-15.

 
 

Fang D, Krueger RR, Roose ML (1998). Phylogenetic relationships among selected Citrus germplasm accessions revealed by inter-simple sequence repeat (ISSR) markers. Journal of the American Society for Horticultural Science 123(4):612-617.

 
 

Felsenstein J (2004). PHYLIP (Phylogeny Inference Package) Version 3.6. (Distributed by the author) Department of Genome Sciences, University of Washington, Seattle.

 
 

Forestry Department (2004). Zonatıon of Karımunjawa natıonal park. Jepara Regency. Central Java Provınce.

 
 

Gosslau A, Chen KY, Ho CT, Li S (2014). Anti-inflammatory effects of characterized orange peel extracts enriched with bioactive polymethoxyflavones. Food Science and Human Wellness 3:26-35.
Crossref

 
 

Hanin M, Ebel C, Ngom M, Laplaze L, Masmoudi K (2016). New insights on plant salt tolerance mechanisms and their potential use for breeding. Frontiers in Plant Science pp. 1-17.
Crossref

 
 

Hernandez JA, Jimenez A, Mullineaux P, Sevilla F (2000). Tolerance of Pea (Pisum sativum L.) to long-term salt stree is associated with antioxidant defences. Plant, Cell and Environment 23(8):853-862.
Crossref

 
 

Hirai M, Mitsue S, Kita K, Kajiura I (1990). A survey and isozyme analysis of wild mandarin, Tachibana (Citrus tachibana (Mak.) Tanaka) growing in Japan. Japanese Society for Horticultural Science 59:1-7.
Crossref

 
 

Hussain S, Luro F, Costantino G, Ollitrault P, Morillon R (2012). Physiological analysis of salt stress behaviour of Citrus species and genera: Low chloride accumulation as an indicator of salt tolerance. South African Journal of Botany 81:103-112.
Crossref

 
 

Hynniewta M, Malik SK, Rao SR (2014). Genetic diversity and phylogenetic analysis of Citrus (L) from north-east India as revealed by meiosis, and molecular analysis of internal transcribed spacer region of rDNA. Meta Gene 2:237-251.
Crossref

 
 

Johnson LA, Soltis DE, Soltis PS (1999). Phylogenetic relationships of Polemoniaceae inferred from 18S ribosomal DNA sequences. Plant Systematics and Evolution 214:65-89.
Crossref

 
 

Jukes TH, Cantor CR (1969). Evolution of protein molecules. In Munro HN, editor, Mammalian Protein Metabolism, Academic Press, New York 3(21):132.

 
 

Karsinah, Sudarsono, Setyobudi L, Aswidinnoor H (2002). Keragaman genetik plasma nutfah jeruk berdasarkan analisis penanda RAPD. Jurnal Bioteknologi Pertanian (In Indonesian) 7(1):8-16.

 
 

Koehler-Santos P, Dornelles ALC, De Freitas LB (2003). Characterization of mandarin citrus germplasm from Southern Brazil by morphological and molecular analyses. Pesquisa Agropecuária Brasileira 38(7):797-806.
Crossref

 
 

Kumar R, Vijay S, Khan N (2013). Comparative nutritional analysis and antioxidant activity of fruit juices of some Citrus spp. Octa Journal of Biosciences 1(1):44-53.

 
 

Kusumanıngrum HP (2008). Karakterisasi alga hijau Dunaliella sp. dan isolat sianobakteria serta deteksi gen DXS penyandi enzim kunci biosintesis karotenoid [Characterization of green microalgae Dunaliella sp. and cyanobacteria isolate and detection of DXS gene encoding key enzyme of carotenoid biosynthetic] [PhD Dissertation]. Yogyakarta: Gadjah Mada Universitas (In Indonesian).

 
 

Kyndt T, Dung TN, Goetghebeur P, Toan HT, Gheysen G (2010). Analysis of ITS of the rDNA to infer phylogenetic relationships among Vietnamese Citrus accessions. Genetic Resources and Crop Evolution 57(2):183-192.
Crossref

 
 

Li X, Xie R, Lu Z, Zhou Z (2010). The origin of cultivated Citrus as Inferred from internal transcribed spacer and chloroplast DNA sequence and amplified fragment length polymorphism fingerprints. Journal of The American Society for Horticultural Science 135:341-350.

 
 

Liu F, Wang L, Gu L, Zhao W, Su H, and Cheng X. (2015). Higher transcription levels in ascorbic acid biosynthetic and recycling genes were associated with higher ascorbic acid accumulation in blueberry. Food Chemistry 188:399-405.
Crossref

 
 

Mahmud MK, Hermana N, Zulfianto A, Rozanna R, Apriyantono NI, Hartati B, Bernardus T (2009). Table of Indonesian food composition. Jakarta. Indonesian Nutritionist Association (In Indonesian).

 
 

Mellidou I, Kanellis AK (2017). Genetic control of ascorbic acid biosynthesis and recycling in horticultural crops. Frontiers in Chemistry Mini Review 5(40):1-8.
Crossref

 
 

Morton CM (2009). Phylogenetic relationships of the Aurantioideae (Rutaceae) based on the nuclear ribosomal DNA ITS region and three noncoding chloroplast DNA regions, atpB-rbcL spacer, rps16, and trnL-trnF. Organisms Diversity and Evolution 9(1):52-68.
Crossref

 
 

Moya JL, Primo-Millo E, Talon M (1999). Morphological factors determining salt tolerance in Citrus seedlings: The shoot to root ratio modulates passive root uptake of chloride ions and their accumulation in leaves. Plant Cell and Environment 22:1425-1433.
Crossref

 
 

Murkute A, Sharma S, Singh SK (2005). Citrus in terms of soil and water salinity: A review. Journal of Scientific and Industrial Research 64(06):393-402.

 
 

Nei M, Kumar S (2000) Molecular evolution and Phylogenetics. Oxford University Press pp. 33-46

 
 

Novelli VM, Cristofani M, Souza A, Machado M (2006). Development and characterization of polymorphic microsatellite markers for the sweet orange (Citrus sinensis L. Osbeck). Genetics and Molecular Biology 29(1):90-96.
Crossref

 
 

Nuryandani, E (2012). Persebaran dan karakterisasi induk jeruk keprok Tawangmangu asli (Citrus reticulata Blanco ssp Tawangmangu) (In Indonesian) 13(1):33-42.

 
 

Penjor T, Mimura T, Matsumoto R, Yamamoto M, Nagano Y (2014). Characterization of limes (Citrus aurantifolia) grown in Bhutan and Indonesia using high-throughput sequencing. Scientific Reports 4:1-9.

 
 

Rafiq S, Kaul R, Sofi SA, Bashir N, Nazir F, Nayik GA (2016). Citrus peel as a source of functional ingredient: A review. Journal of The Saudi Society of Agricultural Sciences 7:1-8.
Crossref

 
 

Rogers SO, Bendich AJ (1987). Ribosomal RNA genes in plants: variability in copy number and in the intergenic spacer. Plant Molecular Biology 9(5):509-520.
Crossref

 
 

Samraj S, Rajamurgugan S (2017). Qualitative and quantitative estimation of bioactive compounds and antioxidant activity in Citrus hystrix. International Journal of Engineering Science and Computing 7(6):13154-13163.

 
 

Shimizu T, Kitajima A, Nonaka K, Yoshioka T, Ohta S, Goto S, Toyoda A, Fujiyama A, Mochizuki T, Nagasaki H, Kaminuma E, Nakamura Y (2016). Hybrid origins of citrus varieties inferred from DNA marker analysis of nuclear and organelle genomes. PLoS ONE 11(11): 1-58.
Crossref

 
 

Singh A, Saini ML, Behl RK (2003). Screening of citrus rootstocks for salt tolerance in semi-arid climates-A review. Tropics 13:53-66.
Crossref

 
 

Spínola V, Mendes B, Câmara JS, Castilho PC (2013). Effect of time and temperature on vitamin C stability in horticultural extracts. UHPLC-PDA vs iodometric titration as analytical methods. LWT - Food Science and Technology 50:489-495.
Crossref

 
 

Sun YL, Kang HM, Han SH, Park YC, Hong SK (2015). Taxonomy and phylogeny of the genus Citrus based on the nuclear ribosomal and its region sequence. Pakistan Journal of Botany 47(1):95-101.

 
 

Syvertsen JP, Melgar JC, García-Sánchez F (2010). Salinity tolerance and leaf water use efficiency in Citrus. Journal of the American Society for Horticultural Science 135(1):33-39.

 
 

Tamura K, Glen S, Daniel P, Alan F, Sudhir K (2013). MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30(12):2725-2729.
Crossref

 
 

Tomascir T, Mah AJ, Nontji A, Moosa MK (1970). The ecology of the Indonesian Seas, Part Two. Hong Kong: Eric Oey, Periplus Editions Ltd pp. 685-686.

 
 

Uchoi A, Malik SK, Choudhary R, Kumar S, Pal D, Rohini MR, Chaudhury R (2017). Molecular markers in assessing genetic variation of Indian citron (Citrus medica L.) cultivars collected from different parts of India. Indian Journal of Biotechnology 16:346-356.

 
 

Uzun A, Yesiloglu T, Aka-Kacar Y, Tuzcu O, Gulsen O (2009). Genetic diversity and relationships within Citrus and related genera based on sequence related amplified polymorphism markers (SRAPs). Scientia Horticulturae 121(3):306-312.
Crossref

 
 

Vijayan K (2009). Approaches for enhancing salt tolerance in mulberry (Morus L) -A review. Review Article. Plant Omics Journal 2(1):41-59.

 
 

Wang X, Xu Y, Zhang S, Cao L, Huang Y, Cheng J, Wu G, Tian S, Chen C, Liu Y, Yu H, Yang X, Lan H, Wang N, Wang L, Xu J, Jiang X, Xie Z, Tan M, Larkin RM, Chen L-L, Ma B-G, Ruan Y, Deng X, Xu X (2017). Genomic analyses of primitive, wild and cultivated citrus provide insights into asexual reproduction. Nature 49(5):765-775.

 
 

White TJ, Burns T, Lee S, Taylor J (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. M.A. Innis. D.H. Gelfand, J.J. Sninsky, T.J. White (Eds.), PCR Protocols: A Guide to Methods and Amplifications, Academic Press, San Diego & London pp. 315-322.

 
 

World Conservation Monitoring Centre (1998). Limnocitrus littoralis. The IUCN Red List of Threatened Species 1998: e.T37412A10051534. 
Crossref

 
 

Wu GA, Terol J, Ibanez V, Lopez-Garcia A, Perez-Roman E Borredá C, Domingo CR, Tadeo FR, Carbonell-Caballero J, Alonso R, Curk F, Du D, Ollitrault P, Roose ML, Dopazo J, Gmitter Jr FG, Rokhsar DS, Talon M (2018). Genomics of the origin and evolution of Citrus. Nature 554:311-316.
Crossref

 
 

Xu Q, Chen L, Ruan X, Chen D, Zhu A, Chen C, Bertrand D, Jiao W, Hao B, Lyon MP, Chen J, Gao S, Xing F, Lan H, Chang J, Ge X, Lei Y, Hu Q, Miao Y, Wang L, Xiao S, Biswas MK, Zeng W, Guo F, Cao H, Yang X, Xu X, Cheng Y, Xu J, Liu J, Luo OJ, Tang Z, Guo W, Kuang H, Zhang H, Roose ML, Nagarajan N, Deng X, Ruan Y (2013). The draft genome of sweet orange (Citrus sinensis). Nature Genetics 45(1):59-66.
Crossref

 
 

Yahata M, Kunitake H, Komatsu H (2017). Morphological characterization and evaluation of reproductive function in a haploid pummelo [Citrus maxima (Burm.) Merr.]. Japan Agricultural Research Quarterly 51(4):293-298.
Crossref

 
 

Yamaji H, Kondo K, Kuniga T, Nesumi H, Yoshida T, Hashimoto K, Takheda O (2013). Origin of cultivated Citrus (Rutaceae) documented by the contents of internal transcribed spacer sequences (ITS) in nuclear ribosomal DNA. Journal of Japanese Botany 88:222-238.

 
 

Ywassaki LA, Canniatti-Brazaca SG (2011). Ascorbic acid and pectin in different sizes and parts of citric fruits. Ciência e Tecnologia de Alimentos 31(2):319-326.
Crossref

 
 

Zhang C, Liu J, Zhang Y, Cai X, Gong P, Zhang J, Wang T, Li H, Ye Z. (2011). Overexpression of SlGMEs leads to ascorbate accumulation with enhanced oxidative stress, cold and salt tolerance in tomato. Plant Cell Report 30(3):389-398.
Crossref