International Journal of
Livestock Production

  • Abbreviation: Int. J. Livest. Prod.
  • Language: English
  • ISSN: 2141-2448
  • DOI: 10.5897/IJLP
  • Start Year: 2009
  • Published Articles: 263

Full Length Research Paper

Influence of breed on motility and motion characteristics of fresh, chilled and frozen bull spermatozoa

Kefelegn Seyoum
  • Kefelegn Seyoum
  • National Animal Genetic Improvement Institute, Addis Ababa, Ethiopia.
  • Google Scholar
Alemayehu Lemma
  • Alemayehu Lemma
  • Department of Clinical Studies, Addis Ababa University, College of Veterinary Medicine and Agriculture, Ethiopia.
  • Google Scholar
Asrat Tera
  • Asrat Tera
  • Department of Clinical Studies, Addis Ababa University, College of Veterinary Medicine and Agriculture, Ethiopia.
  • Google Scholar


  •  Received: 23 April 2020
  •  Accepted: 02 November 2020
  •  Published: 31 March 2021

Abstract

At present, frozen-thawed semen is used extensively for artificial insemination (AI) in Ethiopia. However, subjective semen quality assessment is still practiced by the semen processing organizations of the country. In this study, motility and motion characteristics of spermatozoa were assessed by using integrated semen analysis system (ISAS) to diagnose breed differences in quality of semen. Semen was collected from 14 breeding bulls (Boran = 4, Crosses of 75 % Holstein Frisian ËŸ 25% Boran = 4 and Holstein Frisian = 6). After initial subjective assessment, the semen was evaluated at fresh, chilled and frozen stages for various sperm motion characteristics using integrated semen analysis system (ISAS® v1, Proiser, Spain). PH, volume and morphological defects were differed significantly (P < 0.05) among breeds. Significantly higher (P < 0.05) motility percentage (82.5%) was identified in Boran fresh semen. In contrast spermatozoa motion characteristics: Medium, medium progressive and slow types were significantly higher (P < 0.05) in crosses. Individual motility percentage and spermatozoa motion types (medium and slow) were significantly low (P < 0.05) in Boran chilled semen. In line with chilled semen significantly high motility percentage (42.9%) for frozen semen was observed in HF. The sperm kinematic parameters: Average path velocity (VAP, µm/s), straight line velocity (VSL, µm/s), curvilinear velocity (VCL, µm/s), amplitude of lateral head displacement (ALH, µm), beat cross frequency (BCF, Hz) and straightness (STR) percentage were differed significantly (P < 0.05) among the three breeds at all stages of semen production. The smallest kinematic values of all parameters except for LIN, STR and WOB of fresh semen were recorded in cross breed bulls at all stages of production. On the other hand significantly higher (P < 0.05) values for all the parameters (VCL, VAP, VSL, ALH, BCF, LIN, STR and WOB) of frozen semen were recorded in Boran breed. Thus it is possible to conclude that breed has influence on motility and motion characteristics of bull spermatozoa at different stages of semen production.

Key words: Boran, Integrated Semen Analysis System (ISAS), kinematic parameters.