International Journal of
Livestock Production

  • Abbreviation: Int. J. Livest. Prod.
  • Language: English
  • ISSN: 2141-2448
  • DOI: 10.5897/IJLP
  • Start Year: 2009
  • Published Articles: 287

Full Length Research Paper

Six novel PCR-RFLP loci in milk quality candidate genes in Bubalus bubalis

R. Javed1,2, S. K. Gautam2, R. K. Vijh1 and M. S. Tantia1*
  1National Bureau of Animal Genetic Resources, Karnal, Haryana, India. 2Department of Animal Biotechnology, Kurukshetra University, Kurukshetra, Haryana, India.
Email: [email protected]

  •  Accepted: 28 March 2011
  •  Published: 30 June 2011



Prolactin receptor (PRLR) and Peroxisome Proliferator Receptor Gamma Coactivator 1 Alpha (PPARGC1A) are candidate genes associated with milk protein and fat yield in dairy cattle. These genes were sequence characterized to identify exonic SNPs in water buffalo (Bubalus bubalis). Six novel buffalo specific SNPs were identified and novel PCR-RFLP loci for these SNPs were developed and validated. These loci can effectively be used for genotyping PRLR and PPARGC1A genes in recorded buffalo population for association studies with milk protein and fat yield.


Key words: Bubalus bubalis, PRLR gene, PPARGC1A gene, PCR-RFLP, genotyping.