Journal of
AIDS and HIV Research

  • Abbreviation: J. AIDS HIV Res.
  • Language: English
  • ISSN: 2141-2359
  • DOI: 10.5897/JAHR
  • Start Year: 2009
  • Published Articles: 270

Full Length Research Paper

Serum protein electrophoresis as an added value for disease control among HIV patients in a hospital setting in Cameroon

Djuidje Ngounoue Marceline
  • Djuidje Ngounoue Marceline
  • Department of Biochemistry, Faculty of Science, University of Yaounde I, Cameroon.
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Wotchoko Siakam Justin
  • Wotchoko Siakam Justin
  • Department of Biochemistry, Faculty of Science, University of Yaounde I, Cameroon.
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Koanga Mogtomo Martin Luther
  • Koanga Mogtomo Martin Luther
  • Department of Biochemistry, Faculty of Science, University of Douala, Cameroon
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Penda Ida Calixte
  • Penda Ida Calixte
  • Department of Biochemistry, Faculty of Science, University of Douala, Cameroon
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Ngono Ngane Annie Rosalie
  • Ngono Ngane Annie Rosalie
  • Department of Biochemistry, Faculty of Science, University of Douala, Cameroon.
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  •  Received: 05 August 2018
  •  Accepted: 18 October 2018
  •  Published: 30 November 2018

Abstract

In the resource-limited countries, CD4 count and viral load (VL) determination are not always systematic, due to the low follow-up coverage and/or limited access to optimal hospital facilities. The present study aimed at evaluating the clinical role of Serum Protein Electrophoresis (SPE) in HIV health care and disease control. An analytic study was conducted in a Cameroon reference’s hospital, in HIV patients on antiretroviral treatment. Blood sample was collected to perform CD4 count, viral load, total proteins, and SPE through clinical capillary electrophoresis. SPE data were correlated to other results. ANOVA, Mann-Whitney test and Pearson’s correlation were performed, at the threshold P<0.05. A total of 72 patients: 51 (70.8%) women and 21 (29.1%) men were included, with a mean age of 41.22±8.48 years. The electrophoretic profile showed that the majority of patients have hyperproteinemia (70.9%) and hyper-γ-globulinemia (89%). All the enrolled patients had low albumin/globulin ratio. A negative and significant correlation was observed between CD4 count and total proteins (r=-0.5, P<0.0001), and γ-globulin (r=-0.6, P<0.0001). The correlation was positive and significant with the albumin/globulin ratio (r=0.6, P<0.0001). Patients with VL≥250 Cp/mL, had a high total proteins and different fractions of globulins, compared to those with VL<250 Cp/mL. The perturbation of CD4 count and VL were associated with SPE data variations, which better describes patient's clinical status. SPE could be combined to the existing analyses to improve AIDS health care.

Key words: PLHIV, HIV/AIDS, serum proteins electrophoresis, disease control.