Journal of
Brewing and Distilling

  • Abbreviation: J. Brew. Distilling
  • Language: English
  • ISSN: 2141-2197
  • DOI: 10.5897/JBD
  • Start Year: 2010
  • Published Articles: 33

Full Length Research Paper

Construction of bottom-fermenting yeasts that over express the ubiquitin ligase RSP5 gene and their effects on high-gravity fermentation

Tomoo Ogata1*, Hiromi Yamagishi1, Ken Ukibe2 and Hiroshi Takagi2
  1Research Laboratories for Brewing, Asahi Breweries, LTD., Ibaraki, Japan. 2Graduate School of Biological Sciences, Nara Institute of Science and Technology, Nara, Japan.
Email: [email protected]

  •  Accepted: 03 December 2011
  •  Published: 28 February 2012



It has been reported that over expression of the ubiquitin ligase RSP5 gene conferred tolerance to various stresses in laboratory yeast Saccharomyces cereivisiae. We constructed strains of bottom-fermenting yeast, natural hybrid, in which the S. cerevisiae(SC)-RSP5 or Saccharomyces bayanus (SB)-RSP5 is highly expressed and used for high-gravity fermentation. The SB-RSP5-overexpressing yeast showed a higher fermentation rate than the parent strain. In contrast, the fermentation rate of the SC-RSP5-overexpressing yeast was slower than the parent strain. These results suggest significant functional differences in brewing between the SC-RSP5 and SB-RSP5 genes.


Key words: High-gravity fermentation, RSP5 gene, stress tolerance, bottom-fermenting yeast, natural hybrid.