African Journal of
Bacteriology Research

  • Abbreviation: J. Bacteriol. Res.
  • Language: English
  • ISSN: 2006-9871
  • DOI: 10.5897/JBR
  • Start Year: 2009
  • Published Articles: 100

Full Length Research Paper

Antibiotic resistance genes in diarrheagenic Escherichia coli (DEC) isolated from livestock organic wastes in Ouagadougou, Burkina Faso

Evariste BAKO
  • Evariste BAKO
  • Laboratoire de Biologie Moléculaire d’Epidémiologie et de Surveillance des Bactéries et Virus Transmis par les Aliments (LaBESTA), Centre de Recherche en Sciences Biologiques, Alimentaires et Nutritionnelles (CRSBAN), Université Ouaga I Prof Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Asseta KAGAMBEGA
  • Asseta KAGAMBEGA
  • Laboratoire de Biologie Moléculaire d’Epidémiologie et de Surveillance des Bactéries et Virus Transmis par les Aliments (LaBESTA), Centre de Recherche en Sciences Biologiques, Alimentaires et Nutritionnelles (CRSBAN), Université Ouaga I Prof Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Germaine MINOUNGOU
  • Germaine MINOUNGOU
  • Laboratoire National d'Élevage (LNE), Ministère de la Santé animale; 03 BP 7026 Ouagadougou 03 Burkina Faso.
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Noah Obeng NKRUMAH
  • Noah Obeng NKRUMAH
  • Department of Medical Laboratory Sciences, School of Biomedical and Allied Health Sciences, P.O. Box KB 143, Accra, Ghana.
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Tounwendsida Serge BAGRE
  • Tounwendsida Serge BAGRE
  • Laboratoire de Biologie Moléculaire d’Epidémiologie et de Surveillance des Bactéries et Virus Transmis par les Aliments (LaBESTA), Centre de Recherche en Sciences Biologiques, Alimentaires et Nutritionnelles (CRSBAN), Université Ouaga I Prof Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Cheikna ZONGO
  • Cheikna ZONGO
  • Laboratoire de Biochimie et d’Immunologie Appliquée (LABIA), Centre de Recherche en Sciences Biologiques Alimentaires et Nutritionnelles (CRSBAN), Université Ouaga I Professeur Joseph KI-ZERBO, 03 BP 7131 Ouagadougou 03, Burkina Faso.
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Oumar TRAORÉ
  • Oumar TRAORÉ
  • Laboratoire de Biologie Moléculaire d’Epidémiologie et de Surveillance des Bactéries et Virus Transmis par les Aliments (LaBESTA), Centre de Recherche en Sciences Biologiques, Alimentaires et Nutritionnelles (CRSBAN), Université Ouaga I Prof Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Réné DEMBÉLÉ
  • Réné DEMBÉLÉ
  • Laboratoire de Biologie Moléculaire d’Epidémiologie et de Surveillance des Bactéries et Virus Transmis par les Aliments (LaBESTA), Centre de Recherche en Sciences Biologiques, Alimentaires et Nutritionnelles (CRSBAN), Université Ouaga I Prof Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Sidi MOCTAR
  • Sidi MOCTAR
  • Laboratoire National d'Élevage (LNE), Ministère de la Santé animale; 03 BP 7026 Ouagadougou 03 Burkina Faso.
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Anne OUEDRAOGO
  • Anne OUEDRAOGO
  • Laboratoire National d'Élevage (LNE), Ministère de la Santé animale; 03 BP 7026 Ouagadougou 03 Burkina Faso.
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Nicolas BARRO
  • Nicolas BARRO
  • Laboratoire de Biologie Moléculaire d’Epidémiologie et de Surveillance des Bactéries et Virus Transmis par les Aliments (LaBESTA), Centre de Recherche en Sciences Biologiques, Alimentaires et Nutritionnelles (CRSBAN), Université Ouaga I Prof Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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  •  Received: 26 May 2018
  •  Accepted: 15 August 2018
  •  Published: 30 September 2018

Abstract

Diarrheagenic Escherichia coli (DEC) are often disseminated through the fecal matter of livestock and waste products including slurry and manure. The study aimed to characterize archived DEC recovered from cattle fecal matter, manure and slurry for quinolone resistance and extended spectrum beta-lactamases (ESBLs) with focus on trends in antimicrobial susceptibility patterns. The susceptibility of the bacteria was tested using standard laboratory procedures. Polymerase chain reaction (PCR) was carried out to detect the presence of qnrA, qnrB, qnrS genes and β-lactamase producing genes (blaESBL) such us blaTEM and blaSHV. About 91% of DEC strains were multidrug resistant (MDR) with non-susceptibility to ≥1 agent in ≥3 antimicrobial classes. The most common resistance was to amoxicillin-clavulanic acid (96.36%), followed by tretacycline (89.09%), ceftazidime (76.36%), and cefotaxime (70.780%). qnrS (18.2%) was the most prevalent quinolone resistant genes, followed by qnrB (7.2%) and qnrA (2%). blaTEM (5.45%) was most prevalent than blaSHV genes (3.6%). blaTEM and blaSHV genes were identified in double or multiple-carrying with qnrS and qnrB, no Beta-lactamase (ESBLs) producing strains were observed. This result highlights the importance of livestock fecal matter, manure, and slurries as a significant public health concern and a repository of antibiotic resistant gene.

 

Key words: Diarrheagenic Escherichia coli (DEC), Livestock's fecal matter, manure, slurry, antibiotics resistance, quinolone resistance genes, blaTEM, blaSHV, Burkina Faso.