A thermodynamic study on the interaction of bovine carbonic anhydrase II, CAII, with cobalt ions was studied by using isothermal titration calorimetry (ITC) at 27 and 37°C in tris buffer solution at pH = 7.5. The heats of Co2++CAII interaction are reported and analysed in terms of the new solvation theory. It was indicated that there are three identical and non-cooperative sites for Co2+. The binding of a cobalt ion is exothermic with dissociation equilibrium constants of 81.306 and 99.126 µM at 27 and 37°C respectively. The binding of cobalt ions can cause some changes in structure of enzyme, which results in a decrease in the activity and stability of the enzyme.
Key words: Bovine carbonic anhydrase, cobalt ion, isothermal titration calorimetry.
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