Mutagenicity of selected polycyclic aromatic hydrocarbons (PAHs)
June 2023
Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous contaminants with a high octanol-water partition coefficient (kow) and are persistent in the environment. PAHs have various modes of action including mutagenicity, carcinogenicity, and teratogenicity. The mutagenic sensitivity to PAHs of single-cell gel electrophoresis (Comet assay) was compared with the Ames reversion Salmonella experiment (using Salmonella...
Characterisation of induced mutagenicity via single-cell gel electrophoresis in RAW264.7 and Caco-2 cells by hydrogen peroxide
February 2022
The need for a complementary short-term mutagenicity bioassay with robust endpoints to the Ames assay has become increasingly crucial to in order to avoid false negative results. The alternative short-term test (STT) used in conjunction with the Ames increases the validity and decreases the number of false positive outcomes. As a result, Caco-2 cells (Human intestinal epithelial cell model) and RAW264.7 cells...
Pancreatic response after treatment with 1,5-Bis(3,5- dimethylpyrazol-1-yl)-3-oxapentane diacetatocopper in rats
June 2015
3,5-Dimethylpyrazole (U-6245) is a hypoglycemic and antilipolytic agent used in the treatment of diabetes mellitus. Complex 1,5-Bis(3,5-dimethylpyrazol-1-yl)-3-oxapentane diacetatocopper was prepared in 2009. This study indicated that 1,5-Bis(3,5-dimethylpyrazol-1-yl)-3- oxapentane-diacetatocopper has hypoglycemic properties by decreasing glucose level in blood but caused histopathological changes in the pancreas rats....
Basic biology and therapeutic application of stem cells in various human and animal diseases
December 2014
Stem cells are a population of precursor cells that are capable of developing into many different cell types in the body. They are unspecialized cells that can give rise to specialized cells and capable of dividing and renewing themselves for long periods. Based on the potency, stem cells can be totipotent, pluripotent, multipotent, oligopotent, unipotent and based on source it can be divided as embryonic, fetal, adult,...
Role of ginger extract and N-acetylcysteine in acute renal tubular necrosis: Histological, immunohistochemical and gene expression study in rats
October 2014
Acute renal failure (ARF) is a syndrome associated with high mortality rate in humans. ARF now known as acute kidney injury (AKI) represents a significant and devastating problem in clinical medicine. This work was performed on adult male albino rats to study the possible protective effects of ginger which is considered as one of the promising medicinal plants and N-acetylcystiene on acute tubular necrosis induced by...
Constitutive activation of AMP-activated protein kinase (AMPK) propel mitochondrial biogenesis
September 2014
Mitochondrial biogenesis is primarily a critical adaptation aimed to balance an increased workload in an attempt to maintain appropriate body perfusion. Until recently, the signaling mechanisms responsible for this response are poorly understood. To examine the role of AMP-activated protein kinase (AMPK), an evolutionarily conserved fuel sensor, in mitochondrial biogenesis, we used constitutively active and antisense...
Fine structure of adrenal gland of Indian wild caught female bat Taphozous kachhensis (Dobson)
February 2014
The Indian wild caught bat, Taphozous kachhensis at Ambai Nimbai (Maharashtra) shows continuous asynchronous breeding, exhibiting a seasonal polyoestry. The adrenal gland of T. kachhensis is made up of adrenocortical and chromaffin tissues. The adrenocortical tissue is comprised of two main types of cells, the steroid cells with abundance of lipid inclusions and eosinophilic cells with eosinophilic granular cytoplasm....
Blockade of Akt/mTOR pathway and enhancement of histone acetylation by PHI to induce cells apoptosis in prostate cancer PC3 cells
March 2013
Mammalian target of rapamycin (mTOR) has merged an important therapeutic target for cancers. Our object is to demonstrate that phenyhexyle isothiocyanate (PHI), a synthetic isothiocyanate, blocked Akt/mTOR signaling in prostate cancer PC3 cell line, in addition to inhibited histone deacetylase to induce cell apoptosis. Apoptotic cells were measured by TUNEL assay. Histone acetylated H3, H4 and the protein of Akt...
Genetic diversity evaluation of Paraschistura bampurensis (Nikolskii, 1900) in Shapour and Berim Rivers (Iran) using microsatellite markers
March 2013
Paraschistura bampurensis is one of the species of genus Paraschistura. It is distributed in Iran and Pakistan freshwater. This paper reports a study carried out on genetic diversity and population structure of P. bampurensis in Shapour and Berim river basins, Iran. 100 samples of 50 fish each were collected from each river and were used. Microsatellite markers have been increasingly used in...
Assessment of genetic diversity in Berberis lycium Royle complex using RAPD markers
January 2013
Genomic diversity among fifty accessions of Berberis lycium Royle complex was studied, using random amplified polymorphic DNA (RAPD) markers. Out of 80 RAPD primers, 28 were polymorphic and showed reproducible results. Total of 11,683 amplicon generated 50 accessions of B. lycium complex with 28 primers. 332 amplification products scored, 284 (85%) were polymorphic and 48 monomorphic. Maximum numbers...
Bone morphogenetic proteins (BMP) 2, 4, 6 and 7 affect ovarian follicular development through regulation of follicle-stimulating hormone receptor (FSHR) and luteinizing hormone receptor (LHR) expression in goat granulosa cells
January 2013
Bone morphogenetic proteins (BMPs), belonging to the transforming growth factor-beta family, are crucial factors in follicular growth and development in the mammalian ovary. In this study, we examined the effects of BMP2, 4, 6 and 7 on goat granulosa cells by in vitro culture. The results showed that BMP2, 4 and 7 increased follicle-stimulating hormone receptor (FSHR) level in goat granulosa cells whereas they...
Molecular characterization of fusion transcripts in prostate cancer patients: A tool having diagnostic implications in Pakistan
March 2011
Substantial fraction of information has been added with reference to fusion transcripts in hematological malignancies. However these fusion transcripts have been characterized in epithelial cells of prostate and are documented to gear up the process of neoplasia. TMPRSS2-ERG and related transcripts are key offenders in the subversion of core biological systems driving oncogenic transformation. In an RT PCR based study,...
Overexpression of Arabidopsis PP2CA2 gene shows phenotypes related to abscisic acid responses
March 2011
In this study we overexpressed PP2CA2 gene in Arabidopsis to provide genetic evidence on this gene function in seed germination and other plant responses. The average seed germination percentage of constitutive expression of AtPP2CA2 under a cauliflower mosaic virus 35S promoter (35S::AtPP2CA2) was lower than that of wild type under 0.3 and 1 uM exogenous abscisic acid (ABA), indicating an increased...
Molecular variants of Bardet-Biedl Syndrome
September 2010
Bardet-Biedl Syndrome (BBS) is a genetically and clinically heterogeneous disorder clinically characterized by obesity, mental retardation, dysphormic extremities (syndactyly, brachydactyly or polydactyly), retinal dystrophy or pigmentary retinopathy, hypogonadism and kidney structural abnormalities or functional impairment. Till now, 14 genes have been identified for BBS on different chromosomes, that is, 11q13 (BBS1),...
Proliferation of Juglans regia L. by in vitro embryo culture
September 2010
One of the new and important ways of plant propagation is micropropagation or tissue culture technique. Using this technique is preferred due to its high propagation of plants in short periods. This research tried to find the optimal culture media for the in vitro embryo growth and proliferation of Persian walnut (Juglans regia L.). In the first stage of this study, the effects of MS, DKW, NGE, WPM culture media...
Page 1 of 1, showing 15 records out of 15 total, starting on record 1, ending on 15
