Journal of
Cancer Research and Experimental Oncology

  • Abbreviation: J. Cancer Res. Exp. Oncol.
  • Language: English
  • ISSN: 2141-2243
  • DOI: 10.5897/JCREO
  • Start Year: 2009
  • Published Articles: 53

Full Length Research Paper

Human natural killer (NK) cell activation by luteolin from Brucea javanica leaves

Norzila Ismail
  • Norzila Ismail
  • Department of Pharmacology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
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Hasmah Abdullah
  • Hasmah Abdullah
  • School of Health Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
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Veronique Seidel
  • Veronique Seidel
  • Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS), University of Strathclyde, 161 Cathedral Street, Glasgow G4 ORE, United Kingdom.
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Dino Rotondo
  • Dino Rotondo
  • Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS), University of Strathclyde, 161 Cathedral Street, Glasgow G4 ORE, United Kingdom.
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  •  Received: 12 November 2012
  •  Accepted: 17 December 2012
  •  Published: 31 December 2018

Abstract

There is growing interest in using natural substances from plants to potentially enhance anticancer activity. A key target in this respect is natural killer (NK) cells in order to generate an anticancer-immune response. Luteolin used in this study was derived from a local plant Brucea javanica leaves extract; through a bioassay-guided fractionation. The expression of interleukin-2 (IL-2) and interferon-γ (IFN-γ) was increased after 20 h treatment of 0.1 µM luteolin in human mixed lymphocytes, which reflects the activation of NK cells. This was further confirmed in co-culture experiments. NK cell-induced K562 target cell death was increased in the presence of luteolin. These results show that luteolin activates NK cells to kill target cells indicating the potential use of luteolin as an anticancer immunostimulant.

Keywords: Luteolin, Brucea javanica leaves, interleukin-2 (IL-2), interferon-γ (IFN-γ), natural killer cells.