Journal of
Horticulture and Forestry

  • Abbreviation: J. Hortic. For.
  • Language: English
  • ISSN: 2006-9782
  • DOI: 10.5897/JHF
  • Start Year: 2009
  • Published Articles: 299

Full Length Research Paper

Ploidy estimation in West Indian gherkin, horned melon and wild Cucumis spp. by flow cytometry

Yuichi Matsumoto1* and Makoto Miyagi2
1United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan. 2Plant Biotechnology Institute, Ibaraki Agricultural Center, Kasama, Ibaraki 319-0292, Japan.
Email: [email protected]

  •  Accepted: 15 March 2012
  •  Published: 01 April 2012

Abstract

The ploidy level of wild Cucumis spp. differs among species and accessions. Therefore, estimations of ploidy levels are important in the breeding of species and accessions of this plant. Flow cytometry is a powerful technique for estimating ploidy level. This study examined the applicability of flow cytometry analysis for estimating ploidy levels in wild Cucumis spp. West Indian gherkin and horned melon. The relative DNA content of these accessions was highly correlated with their ploidy levels. The results suggest that flow cytometry analysis can be used for estimating ploidy levels in these species.

 

Key words: West Indian gherkin, horned melon, Cucumis spp., ploidy level, flow cytometry.