Journal of
Horticulture and Forestry

  • Abbreviation: J. Hortic. For.
  • Language: English
  • ISSN: 2006-9782
  • DOI: 10.5897/JHF
  • Start Year: 2009
  • Published Articles: 271

Full Length Research Paper

In vitro regeneration of Mango (Mangifera indica L.) cv. Baramasi through nucellar embryogenesis

Khair Tuwair Al-Busaidi
  • Khair Tuwair Al-Busaidi
  • Plant Production Research Centre, Fruit Crop Research Section, Directorate General of Agriculture and Livestock Research, Ministry of Agriculture and Fisheries, P. O. Box 50, Seeb, Postal Code 121, Sultanate of Oman.
  • Google Scholar
Manish Shukla
  • Manish Shukla
  • Plant Production Research Centre, Fruit Crop Research Section, Directorate General of Agriculture and Livestock Research, Ministry of Agriculture and Fisheries, P. O. Box 50, Seeb, Postal Code 121, Sultanate of Oman.
  • Google Scholar
Abdullah Hamed Al-Burashdi
  • Abdullah Hamed Al-Burashdi
  • Plant Production Research Centre, Fruit Crop Research Section, Directorate General of Agriculture and Livestock Research, Ministry of Agriculture and Fisheries, P. O. Box 50, Seeb, Postal Code 121, Sultanate of Oman.
  • Google Scholar
Ghaya Sultan Al-Blushi
  • Ghaya Sultan Al-Blushi
  • Plant Production Research Centre, Fruit Crop Research Section, Directorate General of Agriculture and Livestock Research, Ministry of Agriculture and Fisheries, P. O. Box 50, Seeb, Postal Code 121, Sultanate of Oman.
  • Google Scholar
Mohammed Hamed Al-Jabri
  • Mohammed Hamed Al-Jabri
  • Plant Production Research Centre, Fruit Crop Research Section, Directorate General of Agriculture and Livestock Research, Ministry of Agriculture and Fisheries, P. O. Box 50, Seeb, Postal Code 121, Sultanate of Oman.
  • Google Scholar
Basem Saif Al-Kalbani
  • Basem Saif Al-Kalbani
  • Plant Production Research Centre, Fruit Crop Research Section, Directorate General of Agriculture and Livestock Research, Ministry of Agriculture and Fisheries, P. O. Box 50, Seeb, Postal Code 121, Sultanate of Oman.
  • Google Scholar
Hamoud Darwish Al-Hasani
  • Hamoud Darwish Al-Hasani
  • Plant Production Research Centre, Fruit Crop Research Section, Directorate General of Agriculture and Livestock Research, Ministry of Agriculture and Fisheries, P. O. Box 50, Seeb, Postal Code 121, Sultanate of Oman.
  • Google Scholar


  •  Received: 14 April 2016
  •  Accepted: 04 July 2016
  •  Published: 31 August 2016

Abstract

Vegetative propagation via grafting is a common method to propagate mono-embryonic mango (Mangifera indica L.) varieties in Oman which is time consuming and expensive. Standardize in-vitro regeneration protocol in producing true-to-type, disease free and homogeneous high quality plants is prerequisite. Nucellar tissues from immature mango fruits of mono-embryonic cv. Baramasi were used as explants to induce somatic embryogenesis and plantlets regeneration. Several media compositions were evaluated for all the four stages that is, induction, conversion, maturation and germination. Modified Gamborg’s B5 major salts, MS minor salts, iron source and organics were used as basal media with varying hormone concentrations. The highest number of callused explants (12.6) was observed in induction media 1 (IND1) containing 1 mg/l 2, 4-D, 0.25 mg/l BAP, 400 mg/l L-glutamine and 500 mg/l malt extract. About 49.6% callus produced somatic embryos (SEs) and maximum 69.7 SEs were proliferated from each embryogenic callus in conversion media 2 (CM2) having 0.5 mg/l BAP. The highest germination (35.9%) with well-developed shoot, leaves and roots was observed in germination media 5 (GM5) containing 0.1 mg/l IAA, 1 mg/l Kinetin and 0.5 mg/l GA3. About 65% of transplanted plants are still surviving in green house conditions even after 4 months of transfer.

 

Key words:  Nucellar embryogenesis, mono-embryonic, genotype, callus, germination, maturation, induction, basal media.

Abbreviation

2,4-D, 2,4-Dichlorophenoxyacetic acid; ABA, Abscisic acid; BAP, 6-Benzylaminopurine; GA3, Gibberellic acid;  HgCl2, mercuric chloride; IAA, Indole-3-acetic acid; NAA, 1-Naphthaleneacetic acid; PEG, Polyethylene glycol; PVP, Polyvinylpyrrolidone; cv, cultivar; hrs, hours; MS, Murashige and Skoog; IND, Induction media; CM, Conversion media; M, Maturation media; GM, Germination Media.