The chemical constituents of essential oil were obtained from the fresh leaves and flowers of Hyssopus officinalis L. (Lamiaceae) by hydro distillation method analyzed by gas chromatography and gas chromatography mass spectrometry. Hydro distillation method was used to extract the essential oil. Thirty-five compounds were identified, accounting for 92.13% of the total oil with 0.75% (v/w) oil yield in the essential oil of the leaves. The main constituents of the essential oil were Iso pinocamphone (38.47%), Pinocomphone (13.32%), n-decane (8.67%) and Pinocarvone (5.34%). Thirty-six compounds were identified, accounting for 98.68% of the total oil with 1.38% (v/w) oil yield in the essential oil of the flowers. The main constituents of the essential oil were Iso pinocamphone (40.25%), Pinocomphone (14.92%), n-decane (8.63%) and Pinocarvone (6.76%).
Key words: Hyssopus officinalis L., iso pinocamphone, volatile oil, gas chromatography mass spectrometry (GC/MS).
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