Journal of
Horticulture and Forestry

  • Abbreviation: J. Hortic. For.
  • Language: English
  • ISSN: 2006-9782
  • DOI: 10.5897/JHF
  • Start Year: 2009
  • Published Articles: 274

Full Length Research Paper

Comparative genotyping of some Moldavian grapevine cultivars

Baca I.1*, Bivol I.1, Zamorzaeva I.1, Barbacar N.1, Batrinu T.1, Motoc R.2, Ghetea G. L.2, Barbarii L.3, Constantinescu C.3, Iancu D.3 Cornea V.4, and Korol A.5
1Institute of Genetics and Plant Physiology, Academy of Sciences of Moldova, 20 Padurii street, Chisinau, MD-2002, Republic of Moldova. 2Faculty of Biology, University of Bucharest, Aleea Portocalelor 1-3, Sector 6, Bucharest, Romania. 3National Institute of Legal Medicine Mina Minovici”, Vitan-Barzesti 9 street, sector 4, Bucharest, Romania. 4Department of Grapevine Genetic Resources, National Institute for Viticulture and Oenology, 59 Vierul Street, Chisinau, MD-2070, Republic of Moldova. 5Institute of Evolution, University of Haifa, Mount Carmel, Haifa 31905, Israel.
Email: [email protected]

  •  Accepted: 06 June 2011
  •  Published: 31 December 2011

Abstract

Microsatellite loci (SSRs) represent favourite molecular markers for assessing genetic identities and phylogenetic relationships between different grapevine varieties and gene pools. In the present study, a genetic characterization of 54 grapevine cultivars (genitors and their descendants from the collection of National Institute for Viticulture and Oenology, Department of Grapevine Genetic Resources,Chisinau, Moldova) was carried out based on 15 microsatellite markers. These varieties are among the most important cultivars encountered in the Republic of Moldova. The obtained results showed that in some cases, there is a deviation from the allele size range cited in the literature. This situation proves the action of regional evolutive molecular mechanisms which offered, in time, discrete genetic particularities, indicating that these cultivars belong to a distinct gene pool, which differs from other gene pools presented in different geographical regions, already described in the literature.

 

Key words: Microsatellite loci, genotyping, allelic variants, gene pool, local molecular evolution mechanisms.

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