Journal of
Microbiology and Antimicrobials

  • Abbreviation: J. Microbiol. Antimicrob.
  • Language: English
  • ISSN: 2141-2308
  • DOI: 10.5897/JMA
  • Start Year: 2009
  • Published Articles: 166

Full Length Research Paper

Antimicrobial activity of Bacillus cereus: Isolation, identification and the effect of carbon and nitrogen source on its antagonistic activity

Nasser El-Banna
  • Nasser El-Banna
  • College of Science and Health Professions, King Saud bin Abdulaziz University for Health Sciences, King Abdulaziz Medical City, National Guard Health Affairs, Riyadh 11426, Kingdom of Saudi Arabia
  • Google Scholar
Samar S. Qaddoumi
  • Samar S. Qaddoumi
  • National Center for Agricultural Research and Extension (NCARE), Alba’qa, Jordan
  • Google Scholar

  •  Received: 07 April 2015
  •  Accepted: 30 May 2015
  •  Published: 29 February 2016


The present study is concerned with the taxonomy and influences of some cultural conditions on growth and antimicrobial metabolite production of a strain of Bacillus cereus, isolated from Alba’qa, Jordan, and designated NBS1, which is a highly active against a variety of Gram positive bacteria, and less activity was noted against filamentous fungi and yeasts, whereas no activity was detected against tested Gram negative bacteria. In order to optimize the culture conditions for the production of antimicrobial metabolite, the effect of different carbon and nitrogen sources were determined. Effects of nutritional compounds on production of antimicrobial compounds showed that the highest antimicrobial activity was obtained when arabinose and glycerol at 3.5 g/100 (w/v) level was used as sole carbon source. Ca(NO3)2 was identified as a nitrogen source that significantly affected antibiotic production. The results showed that strain NBS1 was a potential soil microorganism with antimicrobial activity and may be used for pharmaceutical and biotechnological purposes.


Key words: Antimicrobial substances, Bacillus cereus, carbon source, fermentation, nitrogen source.