Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3778

Full Length Research Paper

Free radical scavenging and antibacterial activity of essential oil and solvent extracts of Iris planifolia (Mill) from Algeria

Ilyas Chikhi1, Hocine Allali1, Mohamed El Amine Dib1*, Noureddine Halla3, Alain Muselli2, Boufeldja Tabti1 and Jean Costa2
1Laboratoire des Substances Naturelles et Bioactives (LASNABIO), Université Abou Bekr Belkaïd, BP 119, Tlemcen 13000, Algérie. 2Laboratoire Chimie des Produits Naturels, Université de Corse, UMR CNRS 6134, Campus Grimaldi, BP 52, 20250 Corte, France. 3Laboratoire des antibiotiques, antifongique: physique chimie, synthèse et activité biologique, Département de Biologie Moléculaire et Cellulaire, Faculté des Sciences, Université de Tlemcen, 13000, Algérie.
Email: [email protected]

  •  Accepted: 09 January 2012
  •  Published: 16 March 2012


Free radical scavenging and antibacterial activity of essential oil and solvent extracts of Iris planifolia (Mill) was investigated. The essential oil was analyzed by Gas chromatography mass spectrometry (GC-MS) and a total of 38 types of volatile organics were identified. The essential oil consists chiefly of alkanes (36.5%), acids (19.1%), ketones (11.7%), alcohols (9.0%), arylpropanoids (6.8%) and aldehydes (4.1%) accompanied by relatively much smaller amounts of monoterpenes (1.0%). The antimicrobial activity of essential oil and ethanolic extract shows an important activity against Salmonella typhimurium and Klebsiella pneumoniae with minimum inhibitory concentration (MIC) of 3.12 mg/ml. Furthermore, the free radical scavenging assay of the essential oil and extracts were determined using a 2,2-diphenyl-1-picrylhydrazyl (DPPH) test system. The dichloromethane and water extract possessed strong radical scavenging activity with the lowest IC50 value of 0.1 mg/ml followed by the aqueous extract with an IC50 value of 0.12 mg/ml. Phytochemical analysis revealed the presence of flavonoids, terpenoids, saponins, alkalois and tannins which may be responsible for antimicrobial and antioxidant activities.


Key words: Iris planifolia (Mill), essential oil, GC, GC/MS, phytochemical prospecting, antimicrobial and free radical scavenging.